Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/zr1394_6_s456_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 46455148 Number of alignments with a unique best hit from the different alignments: 16197809 Mapping efficiency: 34.9% Sequences with no alignments under any condition: 12629511 Sequences did not map uniquely: 17627828 Sequences which were discarded because genomic sequence could not be extracted: 28969 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 3590424 ((converted) top strand) CT/GA: 3574446 ((converted) bottom strand) GA/CT: 4517598 (complementary to (converted) top strand) GA/GA: 4486372 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 94305654 Total methylated C's in CpG context: 9507171 Total methylated C's in CHG context: 406569 Total methylated C's in CHH context: 5152555 Total methylated C's in Unknown context: 2031 Total unmethylated C's in CpG context: 2409490 Total unmethylated C's in CHG context: 18493047 Total unmethylated C's in CHH context: 58336822 Total unmethylated C's in Unknown context: 16126 C methylated in CpG context: 79.8% C methylated in CHG context: 2.2% C methylated in CHH context: 8.1% C methylated in Unknown context (CN or CHN): 11.2% Bismark completed in 11d 16h 35m 19s