Bismark report for: /home/sam/analyses/20180503_oly_methylseq_trimgalore/zr1394_7_s456_trimmed.fq.gz (version: v0.19.1) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /home/sam/data/oly_methylseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.2 -p 16 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 49730113 Number of alignments with a unique best hit from the different alignments: 16050504 Mapping efficiency: 32.3% Sequences with no alignments under any condition: 13690949 Sequences did not map uniquely: 19988660 Sequences which were discarded because genomic sequence could not be extracted: 32535 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 3651285 ((converted) top strand) CT/GA: 3630795 ((converted) bottom strand) GA/CT: 4389654 (complementary to (converted) top strand) GA/GA: 4346235 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 97950003 Total methylated C's in CpG context: 13411305 Total methylated C's in CHG context: 439668 Total methylated C's in CHH context: 4998131 Total methylated C's in Unknown context: 2679 Total unmethylated C's in CpG context: 2579761 Total unmethylated C's in CHG context: 19668551 Total unmethylated C's in CHH context: 56852587 Total unmethylated C's in Unknown context: 18411 C methylated in CpG context: 83.9% C methylated in CHG context: 2.2% C methylated in CHH context: 8.1% C methylated in Unknown context (CN or CHN): 12.7% Bismark completed in 12d 6h 58m 3s