FastQCFastQC Report
Tue 11 Sep 2018
3_TTAGGC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename3_TTAGGC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11817358
Sequences flagged as poor quality0
Sequence length51
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGGC301.5366022E-647.304081
CAGCCCT252.8985325E-547.304081
GCTGCAT205.501165E-447.304081
TCAGGAC205.501165E-447.304081
CATTAGC205.501165E-447.304081
ACTAGGC205.501165E-447.304081
TCTCCGG205.501165E-447.304081
AACCTGG205.501165E-447.304081
CAATAGC205.501165E-447.304081
CTATGGC650.043.665311
CTCATGG553.6379788E-1143.003711
CTGTAGC451.2427336E-842.0480771
CCTACGT451.2427336E-842.0480771
CTCGTAC402.339657E-741.391071
CCATGTA1600.041.391071
CCTGGAC402.339657E-741.391071
CTGTGGC402.339657E-741.391071
CATAGGC750.040.9968721
ACCGGTC354.4378357E-640.5463561
CATATGC354.4378357E-640.5463561
Produced by FastQC (version 0.11.5)