FastQCFastQC Report
Tue 11 Sep 2018
1_ATCACG_L001_R1_001_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1_ATCACG_L001_R1_001_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9365330
Sequences flagged as poor quality0
Sequence length20-43
%GC24

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATCGT200.00197599436.47701323
TAATTCC200.0020240636.2988519
CCCTGAA200.002064539436.15270212
GCCCTGA200.002064701636.15212211
TCCGTTC200.002064701636.1521228
CCTGAAC200.0020648136.15173713
ACTACGC200.00206535136.1498076
CGAGACT200.002066217136.1467172
CACTAAG250.00559124629.49867430
TGTGCTC3450.029.3364681
GCATATC250.00585962329.21702624
CTTAAGC250.006037165429.0390819
TACGCCC250.00615781728.92178
GTTCCAT250.00615781728.921711
CGTTCCA250.006157977528.92154510
GACGCTC900.028.1141151
GACTTAC350.001015987225.8229479
ACTAAGG502.7271232E-422.17218831
ACGCTCT1201.4551915E-1121.0855852
GTGCTCT5000.020.603632
Produced by FastQC (version 0.11.5)