FastQCFastQC Report
Tue 11 Sep 2018
2_CGATGT_L001_R1_001_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2_CGATGT_L001_R1_001_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11879398
Sequences flagged as poor quality0
Sequence length20-43
%GC24

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTAC556.712071E-1032.7291074
GACTACG601.7062121E-930.002065
CGAGACT601.7062121E-930.0015532
CCTGAAC752.0008883E-1128.80476613
ACTACGC654.0090526E-927.6953756
TGTGCTC11400.027.4751071
CTTAAGC605.3216354E-827.16639519
ACGCCCT708.827556E-925.7187589
CTACGCC708.827556E-925.7186497
CGCCCTG708.827556E-925.71864910
CACTAAG809.622454E-1025.48189530
TACGCCC751.8382707E-824.0041738
GCCCTGA751.8382707E-824.00407211
TTGATCC400.00220527722.6051618
GAGACTA856.931441E-821.1776563
GTGCTCT15350.020.7567752
GACGCTC1650.020.7283461
CGCTCTT1700.020.1187733
CTGAACT1001.7689672E-819.80244314
TGCTCTT15650.019.6688333
Produced by FastQC (version 0.11.5)