FastQCFastQC Report
Tue 11 Sep 2018
4_TGACCA_L001_R1_001_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4_TGACCA_L001_R1_001_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11560658
Sequences flagged as poor quality0
Sequence length20-43
%GC24

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGGC200.001798159237.18451733
CGAACTC200.001864872236.90966830
CATTCCG251.3161398E-436.59856825
CCCTGAA453.3051037E-936.1375612
GGCTGCA200.00206885236.13740510
TCAGGGC200.002069291436.1358413
GACTACG502.3283064E-1036.133655
AGACTAC502.3283064E-1036.1327174
CACTAAG555.14774E-1033.55424530
CGGCCGT250.003163300533.1399336
TACGCCC556.4574124E-1032.852198
CGAGACT556.4574124E-1032.8479232
GCCCTGA509.244104E-932.5239511
ACGCCCT509.244104E-932.5236639
GCATATC601.4533725E-930.4541124
CTTAAGC601.5697879E-930.23639919
CTACGCC601.6389095E-930.11457
ACTACGC601.6407284E-930.1129386
AACTCGG250.005434463729.67088532
GAACTCG250.005504038729.59376131
Produced by FastQC (version 0.11.5)