FastQCFastQC Report
Tue 11 Sep 2018
5_ACAGTG_L001_R1_001_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5_ACAGTG_L001_R1_001_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12502181
Sequences flagged as poor quality0
Sequence length20-43
%GC24

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGC502.2919266E-1036.17793719
CCTGAAC601.8189894E-1235.93107213
AGACTAC556.87578E-1032.660694
TGTGCTC7400.031.314541
GCATATC601.4424586E-930.47772424
GCCCTGA651.2369128E-1030.40637811
ACGCCCT651.2369128E-1030.4062549
CGAGACT601.7444108E-929.9389672
CGCCCTG702.928573E-1028.23437910
CTACGCC654.092726E-927.641947
TTGTTCC450.00803608127.38671137
CACTAAG754.4019544E-1027.25177430
CCCTGAA804.7293724E-1126.95089112
TACGCCC756.511982E-1026.3520878
GTGCTCT9500.024.5814672
GACGCTC2200.024.495521
ACACGAC451.6145474E-423.95644410
GACTACG852.7848728E-923.247385
GATCTCA702.5536428E-723.10076112
TGCTCTT10150.023.0072843
Produced by FastQC (version 0.11.5)