FastQCFastQC Report
Mon 10 Sep 2018
2112_lane1_ACAGTG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_ACAGTG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17327210
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC347139620.034362139086443TruSeq Adapter, Index 5 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT2100661.212347515843578TruSeq Adapter, Index 5 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1809981.0445882516573644TruSeq Adapter, Index 5 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1032460.5958604991801911TruSeq Adapter, Index 5 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC728750.42058127072967894TruSeq Adapter, Index 5 (98% over 50bp)
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC620170.35791682561704974No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC479200.27655923833092577TruSeq Adapter, Index 5 (98% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC434240.2506116102938673TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCGATCTCGTATGC390320.2252641942932532TruSeq Adapter, Index 5 (98% over 50bp)
AACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAA327410.1889571373579474No Hit
GAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCT315290.18196235862553753TruSeq Adapter, Index 5 (100% over 50bp)
CGTAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT239520.13823344900881332TruSeq Adapter, Index 5 (98% over 50bp)
CGCAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT239430.13818150758258255TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGACTCGTATGCC228090.13163688787750594TruSeq Adapter, Index 5 (97% over 40bp)
GTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGT224520.12957654463701887No Hit
GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG211470.12204503783355773No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGAGATCTCGTATGC204440.11798783531797677TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGGGATCTCGTATGC177370.10236500856167842TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)