FastQCFastQC Report
Tue 11 Sep 2018
2112_lane1_ACAGTG_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_ACAGTG_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13408105
Sequences flagged as poor quality0
Sequence length20-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAA917660.6844069314791315TruSeq Adapter, Index 5 (100% over 60bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAA660380.49252299262274574TruSeq Adapter, Index 5 (98% over 63bp)
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC573360.4276219495596134No Hit
GATTGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAA407510.3039281091548731TruSeq Adapter, Index 5 (98% over 63bp)
AACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAA302840.22586338636220404No Hit
GACCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAA301050.22452837295053998TruSeq Adapter, Index 5 (98% over 63bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAA269060.20066966957672244TruSeq Adapter, Index 5 (98% over 63bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAA216870.16174545172490817TruSeq Adapter, Index 5 (98% over 63bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAA212140.158217734720902TruSeq Adapter, Index 5 (100% over 60bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAA203410.15170674752323315TruSeq Adapter, Index 5 (100% over 60bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT186010.13872952218080034TruSeq Adapter, Index 5 (100% over 50bp)
GTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGT183330.1367307311510463No Hit
GACCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC172270.1284819890655689TruSeq Adapter, Index 5 (98% over 50bp)
GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG171540.1279375422552255No Hit
GATTGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAA164430.12263477948599TruSeq Adapter, Index 5 (98% over 63bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAA147840.1102616663577739TruSeq Adapter, Index 5 (98% over 63bp)
TAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTA137300.10240074939747265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)