FastQCFastQC Report
Tue 11 Sep 2018
2112_lane1_CAGATC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_CAGATC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33309531
Sequences flagged as poor quality0
Sequence length20-101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAA1251630.3757573170273697TruSeq Adapter, Index 7 (100% over 60bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAA1106020.33204310201785786TruSeq Adapter, Index 7 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAA656310.19703369585119648TruSeq Adapter, Index 7 (98% over 63bp)
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC435860.13085143708567978No Hit
GAACGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAA431830.12964157315814503TruSeq Adapter, Index 7 (98% over 63bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAA348840.10472678225340369TruSeq Adapter, Index 7 (100% over 60bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)