FastQCFastQC Report
Tue 11 Sep 2018
2112_lane1_GCCAAT_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_GCCAAT_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21885902
Sequences flagged as poor quality0
Sequence length20-101
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA721430.32963229022957335TruSeq Adapter, Index 6 (100% over 60bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA542800.24801353857839625TruSeq Adapter, Index 6 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA292000.1334192211954527TruSeq Adapter, Index 6 (98% over 63bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA260540.1190446708570659TruSeq Adapter, Index 6 (98% over 63bp)
AATAAATCATAAACAAACGGGCAACATACCTAAAACTCAAAAAATTCATC222710.10175957106999749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)