FastQCFastQC Report
Tue 11 Sep 2018
2112_lane1_TGACCA_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_TGACCA_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4207007
Sequences flagged as poor quality0
Sequence length20-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAA947702.2526703663673486TruSeq Adapter, Index 4 (100% over 60bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAA817001.9419981949162433TruSeq Adapter, Index 4 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAA486011.155239342363823TruSeq Adapter, Index 4 (98% over 63bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA454581.0805306480355275TruSeq Adapter, Index 4 (98% over 63bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAA322390.766316766290144TruSeq Adapter, Index 4 (98% over 63bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA265440.630947369471931TruSeq Adapter, Index 4 (100% over 60bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAA264010.6275482783841339TruSeq Adapter, Index 4 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA250390.5951737185129475TruSeq Adapter, Index 4 (98% over 63bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC228230.5424996915859659TruSeq Adapter, Index 4 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAA204140.4852380801838457TruSeq Adapter, Index 4 (100% over 60bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGT195770.4653427008797466TruSeq Adapter, Index 4 (100% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA189740.45100947062840635TruSeq Adapter, Index 4 (98% over 63bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA174610.41504566072744825TruSeq Adapter, Index 4 (98% over 63bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAA173210.41171787924289166TruSeq Adapter, Index 4 (98% over 63bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAA169570.4030656473830445TruSeq Adapter, Index 4 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAA138820.3299733040615335TruSeq Adapter, Index 4 (98% over 63bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA137000.3256471881316099TruSeq Adapter, Index 4 (100% over 60bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA128960.3065362144631564TruSeq Adapter, Index 4 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC125770.29895362665191666TruSeq Adapter, Index 4 (98% over 50bp)
GATCCGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAA119980.28519087322650044TruSeq Adapter, Index 4 (98% over 63bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAA117410.2790820172155644TruSeq Adapter, Index 4 (98% over 63bp)
CGTAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAA107290.2550269110557696TruSeq Adapter, Index 4 (98% over 60bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAA102810.24437801030518846TruSeq Adapter, Index 4 (100% over 58bp)
CGCAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAA99780.23717574037789813TruSeq Adapter, Index 4 (98% over 60bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAA97740.2323266873575442TruSeq Adapter, Index 4 (98% over 63bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAA96270.22883251679875977TruSeq Adapter, Index 4 (100% over 60bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA95930.22802434129536747TruSeq Adapter, Index 4 (98% over 63bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC92700.22034667401314048TruSeq Adapter, Index 4 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA84350.20049883444453503TruSeq Adapter, Index 4 (98% over 63bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA81380.19343918372372568TruSeq Adapter, Index 4 (98% over 63bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA78900.1875442565225111TruSeq Adapter, Index 4 (98% over 63bp)
GATCCGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA71150.16912260901871567TruSeq Adapter, Index 4 (98% over 63bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTT67410.16023267848140021TruSeq Adapter, Index 4 (100% over 59bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA65580.1558827926837298TruSeq Adapter, Index 4 (98% over 63bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC65210.15500330757709696TruSeq Adapter, Index 4 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAA61430.14601829756879417TruSeq Adapter, Index 4 (98% over 63bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTG59190.14069384719350359TruSeq Adapter, Index 4 (98% over 63bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTG59000.14024221970631376TruSeq Adapter, Index 4 (100% over 60bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAA57810.13741360544444067TruSeq Adapter, Index 4 (98% over 63bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA57020.13553578589244086TruSeq Adapter, Index 4 (100% over 60bp)
GAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAA56020.1331587991177576TruSeq Adapter, Index 4 (100% over 58bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAA53640.12750157059401138TruSeq Adapter, Index 4 (98% over 63bp)
GATCCGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA50910.121012396699126TruSeq Adapter, Index 4 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC50740.12060830894742985TruSeq Adapter, Index 4 (100% over 50bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAA48780.11594941486905061TruSeq Adapter, Index 4 (98% over 63bp)
GAGCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC48650.11564040658834178TruSeq Adapter, Index 4 (98% over 50bp)
GATCCGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC46550.11064873436150689TruSeq Adapter, Index 4 (98% over 50bp)
GATCCGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAA43520.10344646443421653TruSeq Adapter, Index 4 (98% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)