FastQCFastQC Report
Tue 11 Sep 2018
2112_lane1_TTAGGC_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_lane1_TTAGGC_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22518331
Sequences flagged as poor quality0
Sequence length20-101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAA699310.3105514347399903TruSeq Adapter, Index 3 (100% over 60bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAA266450.11832582086123523TruSeq Adapter, Index 3 (98% over 63bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAA237720.10556732645949649TruSeq Adapter, Index 3 (98% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)