Bismark report for: /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/whole_genome_BSseq_reads/20180830_trimgalore/2_CGATGT_L001_R1_001_trimmed.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.2 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 11879398 Number of alignments with a unique best hit from the different alignments: 5123570 Mapping efficiency: 43.1% Sequences with no alignments under any condition: 3005093 Sequences did not map uniquely: 3750735 Sequences which were discarded because genomic sequence could not be extracted: 9610 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 2501682 ((converted) top strand) CT/GA: 2497016 ((converted) bottom strand) GA/CT: 58688 (complementary to (converted) top strand) GA/GA: 56574 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 39860399 Total methylated C's in CpG context: 1631498 Total methylated C's in CHG context: 150007 Total methylated C's in CHH context: 987546 Total methylated C's in Unknown context: 1682 Total unmethylated C's in CpG context: 4275154 Total unmethylated C's in CHG context: 7751150 Total unmethylated C's in CHH context: 25065044 Total unmethylated C's in Unknown context: 32864 C methylated in CpG context: 27.6% C methylated in CHG context: 1.9% C methylated in CHH context: 3.8% C methylated in Unknown context (CN or CHN): 4.9% Bismark completed in 0d 2h 53m 19s