Bismark report for: /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/whole_genome_BSseq_reads/20180830_trimgalore/4_TGACCA_L001_R1_001_trimmed.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.2 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 11560658 Number of alignments with a unique best hit from the different alignments: 5038185 Mapping efficiency: 43.6% Sequences with no alignments under any condition: 2902106 Sequences did not map uniquely: 3620367 Sequences which were discarded because genomic sequence could not be extracted: 9284 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 2483585 ((converted) top strand) CT/GA: 2478522 ((converted) bottom strand) GA/CT: 33228 (complementary to (converted) top strand) GA/GA: 33566 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 39013786 Total methylated C's in CpG context: 1647867 Total methylated C's in CHG context: 75737 Total methylated C's in CHH context: 679888 Total methylated C's in Unknown context: 1531 Total unmethylated C's in CpG context: 4125544 Total unmethylated C's in CHG context: 7644415 Total unmethylated C's in CHH context: 24840335 Total unmethylated C's in Unknown context: 32365 C methylated in CpG context: 28.5% C methylated in CHG context: 1.0% C methylated in CHH context: 2.7% C methylated in Unknown context (CN or CHN): 4.5% Bismark completed in 0d 6h 3m 9s