Bismark

Bismark Processing Report

/gscratch/scrubbed/samwhite/data/O_lurida/BSseq/whole_genome_BSseq_reads/20180830_trimgalore/5_ACAGTG_L001_R1_001_trimmed.fq.gz

Data processed at 01:47 on 2018-09-13


Alignment

Sequences analysed in total 12502181
Single-end alignments with a unique best hit 5356293
Sequences without alignments under any condition 3233277
Sequences that did not map uniquely 3912611
Genomic sequence context not extractable (edges of chromosomes) 9923

Cytosine Methylation

Total C's analysed 41733973
Methylated C's in CpG context 1718952
Methylated C's in CHG context 100424
Methylated C's in CHH context 910949
Methylated C's in Unknown context 1864
Unmethylated C's in CpG context 4542008
Unmethylated C's in CHG context 8227037
Unmethylated C's in CHH context 26234603
Unmethylated C's in Unknown context 36333
Percentage methylation (CpG context) 27.5%
Percentage methylation (CHG context) 1.2%
Percentage methylation (CHH context) 3.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2629454 original top strand
CTOT 43160 complementary to original top strand
CTOB 47680 complementary to original bottom strand
OB 2626076 original bottom strand