Bismark report for: /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/whole_genome_BSseq_reads/20180830_trimgalore/5_ACAGTG_L001_R1_001_trimmed.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.2 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 12502181 Number of alignments with a unique best hit from the different alignments: 5356293 Mapping efficiency: 42.8% Sequences with no alignments under any condition: 3233277 Sequences did not map uniquely: 3912611 Sequences which were discarded because genomic sequence could not be extracted: 9923 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 2629454 ((converted) top strand) CT/GA: 2626076 ((converted) bottom strand) GA/CT: 43160 (complementary to (converted) top strand) GA/GA: 47680 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 41733973 Total methylated C's in CpG context: 1718952 Total methylated C's in CHG context: 100424 Total methylated C's in CHH context: 910949 Total methylated C's in Unknown context: 1864 Total unmethylated C's in CpG context: 4542008 Total unmethylated C's in CHG context: 8227037 Total unmethylated C's in CHH context: 26234603 Total unmethylated C's in Unknown context: 36333 C methylated in CpG context: 27.5% C methylated in CHG context: 1.2% C methylated in CHH context: 3.4% C methylated in Unknown context (CN or CHN): 4.9% Bismark completed in 0d 7h 44m 16s