Bismark report for: /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/whole_genome_BSseq_reads/20180830_trimgalore/6_GCCAAT_L001_R1_001_trimmed.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.2 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 12409649 Number of alignments with a unique best hit from the different alignments: 5269110 Mapping efficiency: 42.5% Sequences with no alignments under any condition: 3275362 Sequences did not map uniquely: 3865177 Sequences which were discarded because genomic sequence could not be extracted: 10014 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 2577697 ((converted) top strand) CT/GA: 2575616 ((converted) bottom strand) GA/CT: 55210 (complementary to (converted) top strand) GA/GA: 50573 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 40940100 Total methylated C's in CpG context: 1640749 Total methylated C's in CHG context: 127541 Total methylated C's in CHH context: 942648 Total methylated C's in Unknown context: 1916 Total unmethylated C's in CpG context: 4498550 Total unmethylated C's in CHG context: 8020739 Total unmethylated C's in CHH context: 25709873 Total unmethylated C's in Unknown context: 34838 C methylated in CpG context: 26.7% C methylated in CHG context: 1.6% C methylated in CHH context: 3.5% C methylated in Unknown context (CN or CHN): 5.2% Bismark completed in 0d 9h 24m 9s