Bismark report for: /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/whole_genome_BSseq_reads/20180830_trimgalore/8_ACTTGA_L001_R1_001_trimmed.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.2 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 14269815 Number of alignments with a unique best hit from the different alignments: 6097629 Mapping efficiency: 42.7% Sequences with no alignments under any condition: 3681763 Sequences did not map uniquely: 4490423 Sequences which were discarded because genomic sequence could not be extracted: 11428 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 2996945 ((converted) top strand) CT/GA: 2991839 ((converted) bottom strand) GA/CT: 49453 (complementary to (converted) top strand) GA/GA: 47964 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 47411889 Total methylated C's in CpG context: 1798021 Total methylated C's in CHG context: 112507 Total methylated C's in CHH context: 860463 Total methylated C's in Unknown context: 1784 Total unmethylated C's in CpG context: 5295215 Total unmethylated C's in CHG context: 9386854 Total unmethylated C's in CHH context: 29958829 Total unmethylated C's in Unknown context: 39384 C methylated in CpG context: 25.3% C methylated in CHG context: 1.2% C methylated in CHH context: 2.8% C methylated in Unknown context (CN or CHN): 4.3% Bismark completed in 0d 12h 42m 54s