Bismark report for: /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/whole_genome_BSseq_reads/20180830_trimgalore/2_CGATGT_L001_R1_001_trimmed.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 11879398 Number of alignments with a unique best hit from the different alignments: 6229488 Mapping efficiency: 52.4% Sequences with no alignments under any condition: 1480776 Sequences did not map uniquely: 4169134 Sequences which were discarded because genomic sequence could not be extracted: 15981 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 2964533 ((converted) top strand) CT/GA: 2969424 ((converted) bottom strand) GA/CT: 140820 (complementary to (converted) top strand) GA/GA: 138730 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 48108016 Total methylated C's in CpG context: 1870637 Total methylated C's in CHG context: 202679 Total methylated C's in CHH context: 1823533 Total methylated C's in Unknown context: 26029 Total unmethylated C's in CpG context: 5123537 Total unmethylated C's in CHG context: 9169356 Total unmethylated C's in CHH context: 29918274 Total unmethylated C's in Unknown context: 143090 C methylated in CpG context: 26.7% C methylated in CHG context: 2.2% C methylated in CHH context: 5.7% C methylated in Unknown context (CN or CHN): 15.4% Bismark completed in 0d 3h 30m 32s