Bismark report for: /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/whole_genome_BSseq_reads/20180830_trimgalore/5_ACAGTG_L001_R1_001_trimmed.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 12502181 Number of alignments with a unique best hit from the different alignments: 6565485 Mapping efficiency: 52.5% Sequences with no alignments under any condition: 1566378 Sequences did not map uniquely: 4370318 Sequences which were discarded because genomic sequence could not be extracted: 16973 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 3136437 ((converted) top strand) CT/GA: 3144162 ((converted) bottom strand) GA/CT: 131919 (complementary to (converted) top strand) GA/GA: 135994 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 50755672 Total methylated C's in CpG context: 1984755 Total methylated C's in CHG context: 149001 Total methylated C's in CHH context: 1826039 Total methylated C's in Unknown context: 28729 Total unmethylated C's in CpG context: 5498703 Total unmethylated C's in CHG context: 9800659 Total unmethylated C's in CHH context: 31496515 Total unmethylated C's in Unknown context: 160224 C methylated in CpG context: 26.5% C methylated in CHG context: 1.5% C methylated in CHH context: 5.5% C methylated in Unknown context (CN or CHN): 15.2% Bismark completed in 0d 9h 26m 51s