Bismark report for: /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/whole_genome_BSseq_reads/20180830_trimgalore/7_CAGATC_L001_R1_001_trimmed.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 10253191 Number of alignments with a unique best hit from the different alignments: 5382140 Mapping efficiency: 52.5% Sequences with no alignments under any condition: 1289656 Sequences did not map uniquely: 3581395 Sequences which were discarded because genomic sequence could not be extracted: 14015 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 2588855 ((converted) top strand) CT/GA: 2588882 ((converted) bottom strand) GA/CT: 95465 (complementary to (converted) top strand) GA/GA: 94923 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 41275205 Total methylated C's in CpG context: 1610454 Total methylated C's in CHG context: 98157 Total methylated C's in CHH context: 1236583 Total methylated C's in Unknown context: 21382 Total unmethylated C's in CpG context: 4341462 Total unmethylated C's in CHG context: 7971801 Total unmethylated C's in CHH context: 26016748 Total unmethylated C's in Unknown context: 121435 C methylated in CpG context: 27.1% C methylated in CHG context: 1.2% C methylated in CHH context: 4.5% C methylated in Unknown context (CN or CHN): 15.0% Bismark completed in 0d 13h 10m 16s