Bismark report for: /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/whole_genome_BSseq_reads/20180830_trimgalore/8_ACTTGA_L001_R1_001_trimmed.fq.gz (version: v0.19.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/scrubbed/samwhite/data/O_lurida/BSseq/20180503_oly_genome_pbjelly_sjw_01_bismark/ with the specified options: -q --score-min L,0,-0.6 -p 28 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 14269815 Number of alignments with a unique best hit from the different alignments: 7450760 Mapping efficiency: 52.2% Sequences with no alignments under any condition: 1807284 Sequences did not map uniquely: 5011771 Sequences which were discarded because genomic sequence could not be extracted: 19179 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 3567172 ((converted) top strand) CT/GA: 3566713 ((converted) bottom strand) GA/CT: 149624 (complementary to (converted) top strand) GA/GA: 148072 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 57476895 Total methylated C's in CpG context: 2072002 Total methylated C's in CHG context: 165895 Total methylated C's in CHH context: 1839579 Total methylated C's in Unknown context: 31780 Total unmethylated C's in CpG context: 6356294 Total unmethylated C's in CHG context: 11147692 Total unmethylated C's in CHH context: 35895433 Total unmethylated C's in Unknown context: 174320 C methylated in CpG context: 24.6% C methylated in CHG context: 1.5% C methylated in CHH context: 4.9% C methylated in Unknown context (CN or CHN): 15.4% Bismark completed in 0d 15h 31m 22s