##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	20150521_trimmed_2212_lane2_1000ppm_CTTGTA.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	57867827	
Filtered Sequences	0	
Sequence length	1-51	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.589438048192136	40.0	37.0	41.0	32.0	41.0
2	37.52428975592296	40.0	36.0	41.0	32.0	41.0
3	37.51054220497587	40.0	36.0	41.0	32.0	41.0
4	37.47243460184454	40.0	36.0	41.0	32.0	41.0
5	37.41704575406135	40.0	36.0	41.0	31.0	41.0
6	37.3865201244244	40.0	36.0	41.0	31.0	41.0
7	37.32914312036783	39.0	36.0	41.0	31.0	41.0
8	37.319458765107576	39.0	35.0	41.0	31.0	41.0
9	37.27077721795159	39.0	35.0	41.0	31.0	41.0
10	37.22008932643088	39.0	35.0	41.0	31.0	41.0
11	37.17059071865957	39.0	35.0	41.0	31.0	41.0
12	37.12259784876282	39.0	35.0	41.0	31.0	41.0
13	37.08626861803239	39.0	35.0	41.0	31.0	41.0
14	36.99094633769384	39.0	35.0	41.0	31.0	41.0
15	36.93575375407592	39.0	35.0	41.0	31.0	41.0
16	36.87804171491194	39.0	35.0	41.0	31.0	41.0
17	36.78270691063361	39.0	35.0	40.0	31.0	41.0
18	36.72036929866342	39.0	35.0	40.0	31.0	41.0
19	36.66125036337891	38.0	35.0	40.0	31.0	41.0
20	36.55748182236664	38.0	35.0	40.0	31.0	41.0
21	36.46380785942594	38.0	35.0	40.0	31.0	41.0
22	36.340502003659665	38.0	34.0	40.0	31.0	41.0
23	36.21865402515043	37.0	34.0	40.0	30.0	41.0
24	36.08430713294435	37.0	34.0	40.0	30.0	41.0
25	35.928293737880175	37.0	34.0	39.0	30.0	41.0
26	35.78666873811086	37.0	34.0	39.0	30.0	41.0
27	35.59811948895407	36.0	34.0	39.0	30.0	41.0
28	35.41422434735323	36.0	34.0	39.0	30.0	40.0
29	35.244371049508295	36.0	34.0	39.0	30.0	40.0
30	35.070655721794076	36.0	34.0	39.0	30.0	40.0
31	34.916090122040316	35.0	34.0	37.0	30.0	39.0
32	34.74064467695759	35.0	34.0	37.0	30.0	39.0
33	34.563246229250666	35.0	34.0	37.0	30.0	39.0
34	34.4144532794314	35.0	33.0	37.0	30.0	39.0
35	34.27011754620353	35.0	33.0	37.0	30.0	39.0
36	34.06213262185093	35.0	33.0	36.0	29.0	38.0
37	30.99812843414625	32.0	29.0	34.0	25.0	36.0
38	32.656539735181745	34.0	31.0	35.0	27.0	36.0
39	33.35521520331476	35.0	33.0	35.0	29.0	37.0
40	33.55689952488008	35.0	33.0	35.0	29.0	37.0
41	33.598881461617545	35.0	33.0	35.0	29.0	37.0
42	33.563417178950445	35.0	33.0	35.0	30.0	36.0
43	33.51451002473397	35.0	33.0	35.0	30.0	36.0
44	33.45471558148487	35.0	33.0	35.0	30.0	36.0
45	33.4009014173105	35.0	33.0	35.0	29.0	36.0
46	33.373722179073475	35.0	33.0	35.0	30.0	36.0
47	33.327357035429586	35.0	33.0	35.0	29.0	36.0
48	33.275785170328334	35.0	33.0	35.0	29.0	35.0
49	33.240432526296196	35.0	33.0	35.0	29.0	35.0
50	33.2093188730504	35.0	33.0	35.0	29.0	35.0
51	33.34761124246944	35.0	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	55.0
14	484.0
15	10748.0
16	12896.0
17	20850.0
18	30306.0
19	40629.0
20	53444.0
21	78197.0
22	118565.0
23	186194.0
24	294454.0
25	448953.0
26	654326.0
27	901269.0
28	1184653.0
29	1502944.0
30	1868559.0
31	2323568.0
32	2960475.0
33	3910010.0
34	5252200.0
35	5961185.0
36	7195514.0
37	9862954.0
38	9507040.0
39	3098305.0
40	378327.0
41	10723.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	10.410888592723552	35.42058698696255	21.257618676436564	32.910905743877336
2	10.381823991752803	36.42998381217218	21.54993711474018	31.638255081334837
3	10.213186641753735	35.923523614934915	22.173202489778422	31.69008725353293
4	10.380974579484631	35.452070744045535	21.258354606253707	32.908600070216124
5	10.43549220945168	36.518750609988686	21.5027904966416	31.542966683918035
6	10.179953543325325	35.86342655776537	22.170491718166314	31.78612818074299
7	10.412861979028017	35.391864125072935	21.265151125527105	32.93012277037194
8	10.368023230002722	36.41242046674743	21.61099531598869	31.608560987261157
9	10.202396147530616	36.022915814632775	22.186433715948148	31.58825432188846
10	10.333583759311137	35.527807204489015	21.326305807963873	32.812303228235976
11	10.385899996050412	36.48270763949068	21.616571382641208	31.514820981817703
12	10.145378736737849	35.91449028491185	22.32889461612731	31.611236362222993
13	10.307898306860306	35.414589366361746	21.45100474965648	32.82650757712146
14	10.285700178842415	36.413994751986536	21.764177212752912	31.53612785641814
15	10.1552130593686	35.96925518459155	22.355367077635453	31.520164678404395
16	10.26056723524035	35.483576007328196	21.482031273013874	32.77382548441758
17	10.353027818648265	36.47339168267938	21.652969909681783	31.52061058899057
18	10.09510838737238	35.95367608352342	22.285358164870516	31.66585736423368
19	10.279719036339499	35.50347256555095	21.45512028866729	32.76168810944227
20	10.246925694866391	36.444242788592206	21.763487092310744	31.54534442423066
21	10.10584493407887	36.013697053608766	22.339608883852474	31.54084912845989
22	10.212279880652375	35.55542957547519	21.504485465398727	32.72780507847371
23	10.273419085218396	36.45109403985461	21.744492012539933	31.53099486238706
24	10.015687451383362	35.89457916041745	22.454198018036724	31.635535370162458
25	10.199195475047224	35.48481117660435	21.5915295535676	32.724463794780824
26	10.182703658818529	36.3978614061721	21.865480992622143	31.55395394238723
27	10.00240555470776	35.9790986359693	22.395368245380496	31.62312756394244
28	10.148251536994517	35.537046754926635	21.544442158044433	32.77025955003442
29	10.161461240661913	36.477127849271156	21.79456067208461	31.56685023798232
30	9.97207975193946	35.93421881035198	22.41471969587038	31.678981741838175
31	10.10028671786139	35.612263087680034	21.583551903991836	32.703898290466746
32	10.083202246947582	36.49653552250719	21.892874435632073	31.527387794913153
33	9.908089633781193	36.01833459604631	22.469815745563917	31.603760024608583
34	10.054652570709841	35.56217438013448	21.65342751889464	32.72974553026104
35	10.059961293005657	36.49655136194652	21.88977461705294	31.55371272799488
36	9.85398291995835	35.92156373741049	22.49110083560681	31.73335250702435
37	9.946977071919703	35.63967946377971	21.710646627508645	32.70269683679195
38	9.955894430413114	36.5107753705826	21.94316655002467	31.590163648979612
39	9.81424460472368	36.061850555491105	22.47280927871515	31.651095561070065
40	9.943416055266995	35.66110280743126	21.69386629781374	32.701614839487995
41	9.982849963791194	36.533657338754196	21.90494015289474	31.57855254455987
42	9.776317087014268	36.012015295304664	22.522619914457888	31.68904770322318
43	9.9151894022142	35.64708439731936	21.75479834799475	32.682927852471686
44	9.91680629156389	36.53102712461697	22.020988633722123	31.531177950097018
45	9.781548251918322	36.07586609005279	22.560780642485884	31.581805015543008
46	9.889864873815664	35.71322495828363	21.746414551492048	32.65049561640865
47	9.874180104398931	36.91848354695573	21.90568673037522	31.301649618270122
48	10.229113446441145	36.20850827079763	22.316683521988754	31.245694760772473
49	10.331803736250688	36.24026865250329	21.443657168957984	31.984270442288036
50	10.328443102907855	36.96522089639339	22.09364821719067	30.612687783508086
51	10.165817919701828	36.36261657490536	22.433626439258457	31.037939066134356
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.32179433660089
2	42.020079073087636
3	41.90327389528667
4	43.289574649700754
5	41.97845889336971
6	41.96608172406832
7	43.34298474939996
8	41.976584217263884
9	41.79065046941908
10	43.14588698754711
11	41.90072097786811
12	41.75661509896084
13	43.13440588398177
14	41.82182803526055
15	41.675377737773
16	43.03439271965793
17	41.873638407638836
18	41.76096575160606
19	43.04140714578176
20	41.792270119097054
21	41.64669406253876
22	42.94008495912608
23	41.804413947605454
24	41.65122282154582
25	42.92365926982804
26	41.736657601205756
27	41.625533118650196
28	42.91851108702894
29	41.72831147864423
30	41.65106149377763
31	42.80418500832813
32	41.61059004186074
33	41.51184965838978
34	42.78439810097088
35	41.613674021000534
36	41.5873354269827
37	42.649673908711655
38	41.54605807939273
39	41.46534016579375
40	42.645030894754996
41	41.561402508351065
42	41.46536479023745
43	42.59811725468589
44	41.447984241660905
45	41.36335326746133
46	42.540360490224316
47	41.17582972266905
48	41.47480820721362
49	42.31607417853873
50	40.94113088641594
51	41.20375698583618
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	41980.0
1	41965.0
2	41995.5
3	42554.0
4	44270.0
5	47260.5
6	52884.0
7	60032.0
8	68539.0
9	74790.5
10	83673.0
11	93175.0
12	96445.0
13	111380.5
14	128203.5
15	138557.0
16	148230.5
17	171377.5
18	197831.5
19	234106.5
20	269656.5
21	341776.5
22	416065.5
23	553072.5
24	693787.5
25	956861.5
26	1582896.0
27	1977498.0
28	2493213.5
29	3014149.0
30	3573745.5
31	4140139.5
32	4611301.5
33	5070665.5
34	5328679.0
35	5585909.5
36	5607565.5
37	5615892.5
38	5426178.5
39	5231438.5
40	4930240.0
41	4618990.0
42	4274435.0
43	3924749.0
44	3598449.5
45	3275472.0
46	3016964.0
47	2761728.0
48	2598872.0
49	2438231.0
50	2310693.5
51	2177279.5
52	2074797.5
53	1969014.0
54	1902022.5
55	1839215.5
56	1788542.0
57	1742406.5
58	1689619.5
59	1636015.0
60	1579825.0
61	1520954.5
62	1443795.5
63	1359967.0
64	1267401.5
65	1180332.5
66	1090496.0
67	1004097.0
68	917431.0
69	826153.5
70	737263.5
71	650157.0
72	577818.5
73	505877.0
74	451532.5
75	361315.5
76	310774.5
77	280786.5
78	251275.0
79	226236.5
80	202950.0
81	186777.0
82	170335.0
83	152681.5
84	136639.0
85	129668.5
86	122187.0
87	118373.0
88	105762.5
89	93136.0
90	85719.0
91	78704.5
92	73477.0
93	65946.5
94	59191.0
95	53848.5
96	51098.5
97	49689.0
98	49253.5
99	49229.5
100	49242.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.278944435308441E-4
3	0.0016686039186981471
4	2.665201290538923E-4
5	3.294891601794731E-5
6	7.992808835139682E-5
7	3.39502310852947E-4
8	5.233239556006374E-6
9	3.495672907508987E-6
10	0.001143567170228502
11	9.12387290999487E-5
12	5.9769381949256385E-5
13	0.0
14	0.0
15	0.0
16	0.0
17	1.2423387630636358E-5
18	3.5563310570088045E-6
19	5.8792689973478615E-5
20	0.0
21	0.0030335854035317875
22	7.1692737402031195E-6
23	3.5740600469028034E-4
24	0.0
25	0.0
26	1.8077532512261447E-6
27	0.0
28	0.0
29	5.462799446538295E-6
30	0.0
31	0.0
32	0.0
33	0.0
34	0.001633257497714271
35	8.715862654917041E-5
36	1.1173478363592696E-5
37	0.0
38	0.0019834913042031965
39	0.0012902657709807685
40	3.979498608568312E-5
41	1.903972898392811E-6
42	0.0
43	3.84874656908298E-6
44	6.970107185146609E-5
45	3.4102520556268624E-4
46	0.0
47	0.0
48	0.0
49	3.948518972722506E-6
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	7612.0
2-3	78463.0
4-5	230019.0
6-7	225865.0
8-9	223844.0
10-11	216711.0
12-13	215870.0
14-15	215722.0
16-17	215987.0
18-19	219303.0
20-21	224774.0
22-23	232688.0
24-25	243685.0
26-27	262273.0
28-29	282575.0
30-31	307080.0
32-33	340394.0
34-35	426375.0
36-37	459130.0
38-39	468990.0
40-41	515738.0
42-43	605595.0
44-45	694909.0
46-47	197754.0
48-49	243582.0
50-51	5.0512889E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	65.61724786685858
#Duplication Level	Relative count
1	100.0
2	45.913935480184016
3	32.40420607480641
4	24.672114752693087
5	18.528731034088636
6	14.209789410520901
7	10.573763465439894
8	7.837814669828843
9	5.8690744920993225
10++	25.729348229848274
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGTGG	1667150	28.072369	31.050274	1
GGGGG	666900	24.236992	25.984175	4
GGGTG	1195380	20.128452	22.972113	3
TGGTG	2448040	19.098934	21.339375	3
GGCGG	1534530	17.711245	19.078068	7
GTGGG	1035720	17.440008	18.960598	4
GTGTG	2140275	16.697836	22.899574	1
TGGGG	879005	14.801157	16.091248	9
GTGGT	1888035	14.729929	16.799067	2
GGAGG	1377430	14.090473	14.916147	7
GGTTG	1779730	13.884965	15.394164	3
GTTGG	1676655	13.080802	14.476382	4
GGGGT	768950	12.947993	14.6253195	2
GGTGT	1637035	12.771698	14.230278	1
TGTTG	3527480	12.75092	13.72501	42
GGGCG	1095080	12.639198	13.704639	25
TGTGG	1566785	12.223626	13.460924	4
GCGGG	999035	11.530664	12.489025	5
GGGTT	1391155	10.85341	14.885671	3
GAGGG	1044350	10.683217	11.617266	1
GGGAG	927430	9.487181	10.014297	10
TGGGT	1203920	9.3926525	10.455533	2
TTGGG	1153325	8.997925	10.024115	5
TGTGT	2407615	8.702901	11.5789	6
GTTGT	2396925	8.664259	9.62965	8
GTTTG	2314230	8.365337	9.637457	46
TGGCG	1476105	7.8936377	8.498713	3
TTGGT	2160495	7.8096266	8.640214	2
GATGG	1645935	7.8010845	8.635516	4
GGTGA	1625200	7.70281	8.442287	10
GGGGA	749975	7.671898	8.225767	28
CGGGG	659975	7.617301	8.2287445	16
CGGCG	2047910	7.5065446	8.090735	1
GCTGG	1349370	7.215908	7.983678	1
GGGGC	624625	7.209298	7.800057	6
TTGTG	1990835	7.196349	8.053194	2
GTGTT	1959610	7.0834785	7.8065934	5
TGGTT	1949110	7.0455236	7.813073	5
GCGGC	1907900	6.993343	7.615674	11
GCGCG	1889510	6.925935	7.3258753	3
AGGGG	662025	6.772209	7.3490887	2
GGATG	1381690	6.548668	7.0793486	15
TTTGG	1783960	6.44855	7.015011	7
GGTCG	1147720	6.137562	6.7811213	22
CGGTG	1131850	6.0526953	6.634908	10
GAGGT	1232430	5.8412337	6.5080233	40
GCGGT	1084430	5.799111	6.2560315	17
GGCGC	1562955	5.728959	6.1959977	1
TGAGG	1203835	5.7057047	6.103026	37
GGTTT	1550890	5.6060634	6.1355915	6
TGCGG	1043650	5.581036	5.8590565	1
GTCGG	1032925	5.5236826	6.223159	2
GGCTG	1004385	5.371062	5.83316	12
CGCGG	1461475	5.356987	5.954775	1
AGGTG	1125590	5.334854	5.797502	9
TTGTT	3176980	5.32081	5.522712	41
GGCGT	994040	5.3157406	5.7872734	14
TGATG	2415365	5.304096	5.8814836	3
AGGGT	1117600	5.2969847	7.756057	2
GGTGC	969565	5.184858	5.5383496	11
GTGAG	1072955	5.085385	5.809968	17
GTGGA	1059955	5.02377	5.5172873	5
GTGGC	924145	4.941969	5.5348463	5
TGGAG	1023480	4.8508925	5.1700873	3
GGCGA	1487390	4.8321066	5.244955	20
GCGTG	900940	4.8178782	5.3152766	3
GAGTG	1005515	4.7657456	5.7542214	2
GGCCG	1209240	4.4324284	4.944248	1
TGTTT	2625675	4.397484	4.7751555	42
AGTGG	903935	4.2842965	5.199709	3
TCGGG	795715	4.2551756	4.7321577	15
TTTGT	2531360	4.239525	4.654876	43
CGTGG	784425	4.194801	4.732473	19
GCCGG	1133100	4.1533394	4.5293984	17
GTGCG	769315	4.1139984	4.448177	2
TGGGC	768660	4.1104956	4.4715014	9
GGGAT	856555	4.0597343	4.403179	8
ATGGG	846730	4.0131674	4.4074516	8
CGGGC	1094140	4.010533	4.339549	6
GTTGA	1794710	3.9411497	4.300632	4
TAGGG	830935	3.9383051	6.0264206	1
TTTTG	2326170	3.8958724	4.2645407	6
GTAGG	817170	3.8730645	4.3046064	43
GTGAT	1720205	3.7775378	4.185009	2
TGGGA	784635	3.7188613	3.989138	12
GAGGA	1272910	3.6651495	4.0351663	2
TGCTG	1477470	3.6607065	3.9363317	6
GAAGG	1265315	3.6432807	3.7871382	17
GGTAG	749800	3.5537574	3.7370894	16
GCAGG	1085790	3.5274222	3.754496	9
GAGAG	1222665	3.5204768	4.176428	1
GTTTT	2095775	3.5100064	3.8549201	7
GATGT	1596695	3.5063121	3.8599572	1
GATTG	1570175	3.4480748	3.7874599	9
GTTGC	1375365	3.4077225	5.0079646	47
GGTAT	1549800	3.403332	4.407991	40
GACGG	1034930	3.3621924	3.6105459	2
CGGGT	625015	3.3423376	3.6333523	2
GTATG	1503420	3.301482	4.3111067	41
GGAGT	695655	3.2971315	3.649847	3
CTGGG	610855	3.2666159	3.5386045	8
CGAGG	990515	3.2179008	3.6222596	1
GGAAG	1114740	3.2097235	3.535488	46
AGGCG	977080	3.1742544	3.4454813	15
GGCAG	975525	3.169203	3.4078052	44
ATGGT	1430650	3.1416805	3.4556289	2
AGGAG	1078245	3.1046414	3.339159	45
GCGGA	955575	3.1043909	3.3817565	14
GGGCT	576600	3.0834334	3.3617783	4
AGCGG	946610	3.075266	3.2658	4
GGGTC	565370	3.0233798	3.2825084	3
AGATC	1378065	0.58385587	6.9355297	47
>>END_MODULE