FastQCFastQC Report
Wed 4 Mar 2015
2112_Cv_LSU.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_Cv_LSU.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224881920
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC65250112.9015276105789205TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC52230312.3225659937446284TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC41072721.8264127236195775TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC36237231.6113892126143354TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC32500161.4452100017644816TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC28533491.2688209883658055TruSeq Adapter, Index 6 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGT3383140.15044072907239497TruSeq Adapter, Index 7 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGT2666870.11858979147812328TruSeq Adapter, Index 4 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT2392940.10640873219154301TruSeq Adapter, Index 5 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2370720.10542065809470143TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAG3176800.086.2268453
GACCGGA2998800.085.911451
ACCGGAA3338400.083.1465762
TCCGAAG1335750.075.963143
CGAAGAG1654650.075.531615
GAGCACA57237300.074.205199
CATCGGA1455550.073.945321
AGAGCAC57607300.073.752288
GAAGAGC58406150.073.04556
AAGAGCA58504550.072.828047
GGAAGAG58020950.071.2025455
CGGAAGA56864100.070.36844
ACGGAAG1572050.070.235663
TCGGAAG51627850.069.532853
GATTGGA1925250.068.780911
ATCGGAA52693350.068.067882
ATTGGAA2029200.067.037482
TTGGAAG2045950.066.311713
GATCGGA52473100.066.240561
TGGAAGA2354500.065.679764
Produced by FastQC (version 0.11.2)