FastQCFastQC Report
Mon 9 Mar 2015
2112_Cv_LSU_truseq-rm.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename2112_Cv_LSU_truseq-rm.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156812090
Sequences flagged as poor quality0
Sequence length1-101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTTCTGCTTGAAAAAA140556528.963372658319905No Hit
CGTCTTCTGCTTGAAAAAAA71246414.543425828965101No Hit
CGTCTTCTGCTTGAAAAA38501692.4552756104455975No Hit
CGTCTTCTGCTTGAAAAAAAA29005301.8496851869010864No Hit
CGTCTTCTGCTTGAAAA17387071.1087837678842238No Hit
CGTCTTCTGCTTGAAAAAAAAA11009090.7020561998759152No Hit
CGTCTTCTGCTTG10281370.6556490637934868Illumina Single End Adapter 1 (100% over 13bp)
CGTCTTCTGCTTGAAA5173150.3298948442049335No Hit
CGTCTTCTGCTT5006720.31928150437890346Illumina Single End Adapter 1 (100% over 12bp)
CGTCTTCTGCTTGAAAAAAAAAA3371210.21498406149678892No Hit
CGTCTTCTGCTTGAA2951150.1881965861178178No Hit
CGTCTTCTGCTTGA2725650.17381631735155115No Hit
CGTCTTCTGCT1570840.1001733986199661Illumina Single End Adapter 1 (100% over 11bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA35102400.074.272039
TGCTTGA35515500.073.954528
CTGCTTG36830300.073.4784247
TCTGCTT37490850.073.019836
TTCTGCT37663950.072.893585
TCTTCTG37774500.072.852923
GTCTTCT38066650.072.6033862
CTTCTGC37947650.072.5458454
CGTCTTC38366950.072.17851
ATCGGAA1650450.047.1442694-95
AATCATA12654700.046.7847335
TCGGAAG854100.044.958394-95
TCATAAA13610300.044.7452167
ATCATAA13787300.044.352816
TAAATCA12293950.044.162533
AAATCAT13481700.043.0054664
CATAAAC14323600.042.792418
ATAAATC13215800.040.8847052
GCTTGTA223700.038.311919
TGAAAAA33275850.037.26570512-13
Produced by FastQC (version 0.11.2)