FastQCFastQC Report
Tue 30 Jun 2015
Geo_Pool_F_GGCTAC_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeo_Pool_F_GGCTAC_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104070998
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAG308100.022.3463528
CGGCTCG132650.022.124173
CGCGGAT41100.022.0288581
CGCGGTT37350.020.6682951
CTCCTAT353550.019.7184941
CGAAAGG288000.019.3580423
CCGGCAT105900.017.233881
GCCGGCT88750.016.966761
CGCGCGT26750.016.7449671
TATGCCG205550.016.74086446-47
GTGCGCG254150.016.3660648
CTACCCG201700.016.1043856
AGGACCG219050.015.8691483
TAGGGCC273050.015.26780412-13
TCTTAGG455000.015.1317379
GCCGGTT86300.014.9636921
GCCGGAT179150.014.7623811
TGCGCGA288500.014.5491839
CCCGCTA305850.014.2982028
CGGGCAT195050.013.8521541
Produced by FastQC (version 0.11.2)