FastQCFastQC Report
Tue 30 Jun 2015
Geo_Pool_F_GGCTAC_L006_R1_001_trimmed.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeo_Pool_F_GGCTAC_L006_R1_001_trimmed.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103911639
Sequences flagged as poor quality0
Sequence length20-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAG308050.022.9696258
CGGCTCG129150.022.259243
CGCGGAT43600.020.5060141
CTCCTAT350650.020.2904681
CGAAAGG288050.019.9440083
CGCGCGT26750.019.3500921
CGCGGTT37500.018.5713981
CCGGCAT104100.018.3070531
GCCGGCT91450.016.9288041
CTACCCG200550.016.7667566
TAGGGCC273750.015.66426612-13
TCTTAGG453450.015.635489
CGCGGGG40150.015.5876351
GTGCGCG248950.015.4261548
AGGACCG214750.015.242863
GCCCGCT46350.015.0254021
GCCGGAT169550.014.5427871
CCCGCTA293250.014.0871728
GACTACC252500.013.8743234
TGCGCGA279800.013.859479
Produced by FastQC (version 0.11.2)