FastQCFastQC Report
Thu 2 Jul 2015
Geo_Pool_F_GGCTAC_L006_R1_001_val_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeo_Pool_F_GGCTAC_L006_R1_001_val_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102867131
Sequences flagged as poor quality0
Sequence length20-86
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTGA296850.020.1463453
CCTTAGG380050.018.60817380
GAGGCGC174700.016.12444180
GCCGACT406400.014.724741
GTCCACG375350.014.32421780
CCGACTG428900.014.1549882
CGTTCGT306250.013.74340480
CCCCTAG440700.013.7416525
CCCTAGT463150.013.1457336
AGCCCGC230900.012.94267380
TCCCCTA476150.012.7433134
GGCCTAT258800.012.41407780
CCTAGTA496650.012.1954687
GACTGAC511500.012.0400164
CGACACG323750.011.92053680
GGGGTCT271050.011.7334626
CCCGGGT138450.011.1443079
TAGGACG304900.011.0879272
TCGCGCC227700.010.83573380
GACCCAT569100.010.7609418
Produced by FastQC (version 0.11.2)