FastQCFastQC Report
Tue 30 Jun 2015
Geo_Pool_F_GGCTAC_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeo_Pool_F_GGCTAC_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104070998
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAG329800.026.6721218
CTCCTAT370250.023.8193441
CGCGGAT46300.021.5634981
CGCGGTT40450.020.5683961
CGCGCGT28700.019.7126941
GTGCGCG254050.019.3503288
CTACCCG203400.019.1553556
CCGGCAT115700.019.1484431
TCTTAGG481150.018.327999
TAGGGCC293250.017.59329812-13
TGCGCGA284050.017.3367379
CGAAAGG279800.016.2439291
ATGTGCG346550.016.151276
GACTACC251800.016.047584
TCGAAGG396300.015.8712897
CTATCTC598300.015.4065434
GCCGGCT89250.015.128331
ACTACCC261050.014.9314465
AGGACCG211050.014.59664253
TTAGGGC310200.014.48946310-11
Produced by FastQC (version 0.11.2)