FastQCFastQC Report
Tue 30 Jun 2015
Geo_Pool_F_GGCTAC_L006_R2_001_trimmed.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeo_Pool_F_GGCTAC_L006_R2_001_trimmed.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103294781
Sequences flagged as poor quality0
Sequence length20-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAG327050.026.1289848
CGCGGAT45200.024.5485481
CTCCTAT360400.023.6559871
CGCGGTT39650.021.2542231
GTGCGCG253000.020.299258
CTACCCG191050.019.3724216
CGCGCGT26050.019.0508371
TGCGCGA277100.018.6988899
CCGGCAT101000.018.5419121
GCCGGCT83700.018.4029031
TCTTAGG473100.018.0789559
TAGGGCC286800.017.6169912-13
ATGTGCG348250.016.5528986
CGCGGGG45550.016.54831
CGAAAGG273850.016.3099081
GACTACC244800.015.6811814
TCGAAGG395200.015.4596157
CTATCTC595000.014.9962354
CCGGGAT104300.014.9926381
GCCGGAT169100.014.9508551
Produced by FastQC (version 0.11.2)