FastQCFastQC Report
Thu 2 Jul 2015
Geo_Pool_F_GGCTAC_L006_R2_001_val_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeo_Pool_F_GGCTAC_L006_R2_001_val_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102867131
Sequences flagged as poor quality0
Sequence length20-86
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTGA311900.023.9118063
CCTTAGG401650.022.65096780
GAGGCGC171850.017.91088180
GCCGACT445200.016.7067431
CCGACTG458400.016.3495642
AGCCCGC251700.016.1246880
CCCCTAG434800.014.8614675
GGGGTCT289400.014.5029586
GACTGAC529350.014.3158014
CCCTAGT450550.014.2577216
CGACACG334500.013.66011780
TCCCCTA473100.013.5536084
CCTAGTA489050.013.1717447
GACCCAT602800.012.7090918
AACGTCG359250.012.688929
CCGGAGC308300.012.34713980
GTATTCG454250.012.2268872
TACGCGA314350.011.87902450-51
ACTGACC673200.011.5885335
ACCCATG670900.011.520259
Produced by FastQC (version 0.11.2)