FastQCFastQC Report
Tue 30 Jun 2015
Geo_Pool_M_CTTGTA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeo_Pool_M_CTTGTA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117663240
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC3082060.2619390728999133No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAGCCTGCTAAA2069880.17591560456774774No Hit
AAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGAAGCGGGAAGAG2065150.17551360985810013No Hit
CACGCTTTCACGGTCTGTATTCGTACTGAAAATCAAAATCAAGTGAGCTT2020180.17169168552557282No Hit
CTAACATGTGCGCGAGTCATGGGGTCTTACGAAACCCAAAGGCGCAATGA1913990.16266677681151734No Hit
CTCCTATCTCTTAGGGCCGACTGACCCATGTTCAACTGCTGTTCACATGG1873570.15923154929271027No Hit
GTCGGATTCCCCTGGTCCGTACCAGTTCTAAGTTGACTGTTAGTCGCCGG1653080.14049247666475953No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA1646330.13991880556748224No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA1636460.1390799709407968No Hit
GGACACTGCAAGGATTGACAGATTGAGAGCTCTTTCTTGATTCGGTGGGT1633600.13883690437217266No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA1576990.1340257161030072No Hit
GCTCAATCTCGTGTGGCTAAACGCCACTTGTCCCTCTAAGAAGTTGGCGA1515330.12878533686476762No Hit
GTCAACACCAGGTGACGGCCATCGCAATGCTTTGTTTTAATTAGACAGTC1330880.11310924295472399No Hit
GTCGGAACCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG1328710.1129248183204882No Hit
GTCAGAACTACGACGGTATCTGATCGTCTTCGAACCTCTGACTTTCGTTC1308940.11124459941779608No Hit
GTGAACTATGCCTGAGTAGGACGAAGTCAGAGGAAACTCTGATGGAGGTC1279390.10873319483638222No Hit
GGAGGACCGACCGATTCGCCACTGACCCTAGGCGTTCCCAGGCGTACGAG1277290.10855471938389595No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC1264010.10742607461769707No Hit
GCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAG1222380.10388801124293365No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG1213620.1031435136411338No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACGGGACTCTTTCGAGGCCCCG1190240.10115648693678672No Hit
GCAGAAATCACATTGCGTCAACACCAGGTGACGGCCATCGCAATGCTTTG1180830.10035674693302683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGT30700.054.9460371
CTCCTAT368950.053.133481
GCCGGCT74450.049.923081
CGCGGTT39000.045.940391
GCCGGAT105950.036.024921
CTCTTAG595200.035.6198738
TCTTAGG605450.035.0405359
GCCAGTA482050.034.3901331
AGTAGTC479400.033.7098244
CCAGTAG480250.033.511152
TGCGCGA586950.033.2973829
CTCCTAG66900.033.1918371
GTGCGCG589300.033.1644488
ATGTGCG619750.031.891736
ATCGAAA266200.031.8806631
CCGGCAT82800.031.6521951
CAGTAGT517200.031.309853
CTACCCG361700.031.0772296
CTAACAT661700.030.022181
GAGACTA388550.029.1247542
Produced by FastQC (version 0.11.2)