FastQCFastQC Report
Thu 2 Jul 2015
Geo_Pool_M_CTTGTA_L006_R1_001_val_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeo_Pool_M_CTTGTA_L006_R1_001_val_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116114671
Sequences flagged as poor quality0
Sequence length20-86
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAGCCTGC3022900.26033747277292807No Hit
TGTATTCGTACTGAAAATCAAAATCAAGTGAGCTTTTGCCCTTTTGCTCT2200060.18947304255807607No Hit
AATTCCGATAACGAACGAGACTCTAGCCTGCTAAATAGTTCGAGGATCCT2012120.17328731870583347No Hit
GCCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCTCCACTT2012070.17328301261775955No Hit
GATTCCCCTAGTAACGGCGAGTGAAGCGGGAAGAGCCCAGCACCGAATCC2004280.17261212409584314No Hit
GTCATGGGGTCTTACGAAACCCAAAGGCGCAATGAAAGTGAAGGCCGCCT1860730.16024934523562487No Hit
CAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCC1845200.15891187427986597No Hit
TCCGTACCAGTTCTAAGTTGACTGTTAGTCGCCGGACGAAGCGGCAGCGC1683730.14500579345395553No Hit
GCTAAACGCCACTTGTCCCTCTAAGAAGTTGGCGAGGACGCATAAGAGGA1660910.14304049485701942No Hit
GTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAGCCTG1606430.13834858129167849No Hit
TGACAGATTGAGAGCTCTTTCTTGATTCGGTGGGTGGTGGTGCATGGCCG1581160.13617228437912038No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACAAACCTTCACATGG1559420.13429999728458086No Hit
CGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCTGGTCC1336550.11510604030389923No Hit
GTATCTGATCGTCTTCGAACCTCTGACTTTCGTTCTTGATTAATGAAAAC1331570.11467715393173701No Hit
GGAGTGTGTAACAACTCACCTGCCGAATCAACTAGCCCTGAAAATGGATG1296960.11169647976697104No Hit
TCGCCACTGACCCTAGGCGTTCCCAGGCGTACGAGTCCGTGCGCCGCAGA1270930.10945473031568939No Hit
CGTCAACACCAGGTGACGGCCATCGCAATGCTTTGTTTTAATTAGACAGT1266410.10906545995380722No Hit
GTAGGACGAAGTCAGAGGAAACTCTGATGGAGGTCCGTAGCGATTCTGAC1261660.108656381586785No Hit
GAAAAATAACAATACGGGACTCTTTCGAGGCCCCGTAATTGGAATGAGTA1198850.10324707374832935No Hit
TGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAA1196090.10300937768664908No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCTA1170140.1007745179762857No Hit
TTGCGCCCCGCTCTCCGCTTAACTGAATAAGTAAAGAAACGATAAAAGTA1162700.10013377207088671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTGA515050.034.457683
CCTTAGG905350.032.26144880
TTGTCTC496400.027.30772
GGGGTCT590850.027.2430256
TGGGGTC622400.025.883835
CTTGTCT532850.025.4538361
GGTCTTA633050.025.3776198
ACCCATG707700.025.1071789
GTCTTAC641050.024.9574979
GTCATGG647250.024.8744661
GTCTCAA593150.024.735614
CTTAGTC77550.024.3318351
GTCCACG880550.024.21998480
ACCCTTG334250.022.62893980
CTCAAAG606050.022.3140986
GACCGAT241450.022.0745451
AAAACTC1098250.022.06519780
GTATTCG876700.022.0404382
TATTCGT897450.021.509313
TCGCCAC491100.021.4332351
Produced by FastQC (version 0.11.2)