FastQCFastQC Report
Tue 30 Jun 2015
Geo_Pool_M_CTTGTA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeo_Pool_M_CTTGTA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117663240
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC2883220.24503999719878528No Hit
CACGCTTTCACGGTCTGTATTCGTACTGAAAATCAAAATCAAGTGAGCTT2579330.21921289945780859No Hit
CTAACATGTGCGCGAGTCATGGGGTCTTACGAAACCCAAAGGCGCAATGA2406300.20450737205604744No Hit
CTCCTATCTCTTAGGGCCGACTGACCCATGTTCAACTGCTGTTCACATGG2362260.20076448685247833No Hit
AAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGAAGCGGGAAGAG2161010.18366058932254456No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA1907800.1621406991682364No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAGCCTGCTAAA1769750.15040806287503217No Hit
GGACACTGCAAGGATTGACAGATTGAGAGCTCTTTCTTGATTCGGTGGGT1567240.13319708007360667No Hit
GTCGGATTCCCCTGGTCCGTACCAGTTCTAAGTTGACTGTTAGTCGCCGG1560490.1326234089763294No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA1550710.13179222329760765No Hit
CCAGAATCGAGGAGCTTGCGCCCCGCTCTCCGCTTAACTGAATAAGTAAA1541180.1309822846965628No Hit
CAAGGATTGACAGATTGAGAGCTCTTTCTTGATTCGGTGGGTGGTGGTGC1516540.128888172720724No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA1496430.12717905779239125No Hit
GCTCAATCTCGTGTGGCTAAACGCCACTTGTCCCTCTAAGAAGTTGGCGA1449280.12317185894252104No Hit
CAGAAATCACATTGCGTCAACACCAGGTGACGGCCATCGCAATGCTTTGT1402090.11916126056022254No Hit
CGCGTGTGCGGCCCCGAACATCTAAGGGCATCACAGACCTGTTATTGCTC1373700.11674844241922966No Hit
CGAGACTACCCGCTGAATTTAAGCATATCAATAAGCGGAGGAAAAGAAAC1318380.11204688907087718No Hit
CGCAAGTCGAAATTGAAATACCACTACTTTTATCGTTTCTTTACTTATTC1282610.10900685719686114No Hit
CGAAAGGGAATCGGGTTAATATTCCCGAACCTGGACACGGAGATCGACTC1248510.10610875580172703No Hit
CGAAGCGGGACTGGTACGGGCACGGGTTGGGCGAGGCCTCACGGTCGAGC1229140.104462532223318No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACGGGACTCTTTCGAGGCCCCG1224060.10403079160492265No Hit
GCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAG1199120.1019111831358715No Hit
GCAGAAATCACATTGCGTCAACACCAGGTGACGGCCATCGCAATGCTTTG1177370.10006268737797805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGT36300.056.056481
CTCCTAT427950.055.5809671
GCCGGCT77450.046.468981
CGCGGTT44100.044.6324161
CTCTTAG638850.039.850368
TCTTAGG649950.039.1843579
TGCGCGA607300.038.8475079
GTGCGCG613800.038.4897658
CTACCCG358250.037.4936036
ATGTGCG648000.037.0356456
GAGACTA379550.035.5749932
CGCGGAT65250.035.3386461
CTAACAT693800.034.7700081
CTCCTAG69750.034.0811651
CATGTGC710400.033.676795
CCGGCAT87350.033.038051
AGACTAC410000.032.942183
GCCGGAT108550.032.4108621
GACTACC415950.032.3208474
ACTACCC429250.031.3499095
Produced by FastQC (version 0.11.2)