FastQCFastQC Report
Thu 2 Jul 2015
Geo_Pool_M_CTTGTA_L006_R2_001_val_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameGeo_Pool_M_CTTGTA_L006_R2_001_val_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116114671
Sequences flagged as poor quality0
Sequence length20-86
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAGCCTGC2866190.24684133153165458No Hit
TGTATTCGTACTGAAAATCAAAATCAAGTGAGCTTTTGCCCTTTTGCTCT2801380.24125978017024222No Hit
GCCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCTCCACTT2534980.2183169429124077No Hit
GTCATGGGGTCTTACGAAACCCAAAGGCGCAATGAAAGTGAAGGCCGCCT2351770.2025385749919577No Hit
CAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCC2145970.18481471647971168No Hit
GATTCCCCTAGTAACGGCGAGTGAAGCGGGAAGAGCCCAGCACCGAATCC2073850.1786036150418925No Hit
AATTCCGATAACGAACGAGACTCTAGCCTGCTAAATAGTTCGAGGATCCT1741190.14995434986850198No Hit
TTGCGCCCCGCTCTCCGCTTAACTGAATAAGTAAAGAAACGATAAAAGTA1634560.14077118644206466No Hit
TCCGTACCAGTTCTAAGTTGACTGTTAGTCGCCGGACGAAGCGGCAGCGC1575390.13567536181539025No Hit
GCTAAACGCCACTTGTCCCTCTAAGAAGTTGGCGAGGACGCATAAGAGGA1549590.13345342036924862No Hit
GTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTAGCCTG1540130.13263870850566334No Hit
TGACAGATTGAGAGCTCTTTCTTGATTCGGTGGGTGGTGGTGCATGGCCG1532390.13197212607182085No Hit
TGAGAGCTCTTTCTTGATTCGGTGGGTGGTGGTGCATGGCCGTTCTTAGT1504160.12954090874528681No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACAAACCTTCACATGG1486820.12804755740125207No Hit
GTCAACACCAGGTGACGGCCATCGCAATGCTTTGTTTTAATTAGACAGTC1482220.12765139729845162No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGTGTGGCTA1349910.1162566270372501No Hit
AATTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCC1305790.11245693492082495No Hit
CGTCAACACCAGGTGACGGCCATCGCAATGCTTTGTTTTAATTAGACAGT1272940.10962783505626089No Hit
AAATACCACTACTTTTATCGTTTCTTTACTTATTCAGTTAAGCGGAGAGC1257290.10828002948912459No Hit
TAATCATTACCTCGAGTTCCGAAAACCAACAAAATAGAACCGAGGTCCTA1248880.1075557454750916No Hit
GAAAAATAACAATACGGGACTCTTTCGAGGCCCCGTAATTGGAATGAGTA1241680.10693566879244741No Hit
ACGGGCACGGGTTGGGCGAGGCCTCACGGTCGAGCTCGAACCGTCCGCCC1206170.10387748504235093No Hit
TTAATATTCCCGAACCTGGACACGGAGATCGACTCGCAAGGGTCAAGTGC1184270.10199141846597488No Hit
TCGCCACTGACCCTAGGCGTTCCCAGGCGTACGAGTCCGTGCGCCGCAGA1175480.10123440818258012No Hit
CGGCCATCGCAATGCTTTGTTTTAATTAGACAGTCGGATTCCCCTGGTCC1173600.10107249927100082No Hit
GTATCTGATCGTCTTCGAACCTCTGACTTTCGTTCTTGATTAATGAAAAC1164280.10026984445402251No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAGG977300.038.25311380
CGACTGA551550.038.205133
GGGGTCT624950.031.9941526
TGGGGTC651300.030.6803785
GGTCTTA662100.030.1476358
GTCATGG675800.029.5393921
GTCTTAC676100.029.4984579
ACCCATG747250.028.3975899
CTTAGTC78600.028.1201321
TCCTCTA318250.025.4980854
AAAACTC1143000.024.69279380
GTATTCG959000.024.397262
TATTCGT975750.024.0068743
TTGTCTC520900.023.9744992
TAAGCAT471750.023.3868395
CTTGTCT538050.023.2706761
ACCCTTG343800.023.16961380
CACTTAT944350.022.74585580
GAGGCGC567300.022.4098280
GTCCACG850950.022.12734480
Produced by FastQC (version 0.11.2)