##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	OlyOv6_combined_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	200998380
Sequences flagged as poor quality	0
Sequence length	76
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78462282133816	31.0	31.0	34.0	30.0	34.0
2	32.0719133109431	33.0	31.0	34.0	30.0	34.0
3	32.23158930435161	34.0	31.0	34.0	30.0	34.0
4	35.814182318285354	37.0	35.0	37.0	35.0	37.0
5	35.667547778245776	37.0	35.0	37.0	33.0	37.0
6	35.602112957328316	37.0	35.0	37.0	33.0	37.0
7	35.69558584999541	37.0	35.0	37.0	33.0	37.0
8	35.67761825742078	37.0	35.0	37.0	33.0	37.0
9	37.30183254213293	39.0	37.0	39.0	34.0	39.0
10-11	37.28812510329685	39.0	37.0	39.0	33.5	39.0
12-13	37.12649599713192	39.0	37.0	39.0	33.0	39.0
14-15	38.24844635066213	40.0	38.0	41.0	33.0	41.0
16-17	38.06852316421654	40.0	37.0	41.0	33.0	41.0
18-19	37.86389191047212	40.0	37.0	41.0	33.0	41.0
20-21	37.688960582667384	39.0	37.0	40.5	32.0	41.0
22-23	37.68085834074881	39.0	37.0	40.5	32.5	41.0
24-25	37.786683101625	40.0	37.0	41.0	32.0	41.0
26-27	37.71693890269165	40.0	37.0	41.0	32.0	41.0
28-29	37.583398632367086	39.5	37.0	41.0	32.0	41.0
30-31	37.22200672214373	39.0	36.0	41.0	31.0	41.0
32-33	37.167562226123415	39.0	36.0	40.0	31.0	41.0
34-35	37.00443233920592	39.0	36.0	40.0	30.5	41.0
36-37	37.009209944876176	39.0	36.0	41.0	30.0	41.0
38-39	36.73672272383489	39.0	36.0	40.5	30.0	41.0
40-41	36.719934160663385	39.0	36.0	40.0	30.0	41.0
42-43	36.5145592914729	39.0	35.0	40.0	30.0	41.0
44-45	36.385182736298674	39.0	35.0	40.0	30.0	41.0
46-47	36.184974209742386	38.5	35.0	40.0	29.5	41.0
48-49	35.92185944981249	38.0	35.0	40.0	28.5	41.0
50-51	35.67409237825698	38.0	34.0	40.0	28.0	41.0
52-53	35.39132576590916	38.0	34.0	40.0	27.5	41.0
54-55	35.080259940403494	38.0	34.0	40.0	26.5	41.0
56-57	34.79315378810516	38.0	33.5	40.0	26.0	41.0
58-59	34.42457189455955	37.5	33.0	40.0	26.0	41.0
60-61	34.007794535458444	37.0	32.5	40.0	25.0	41.0
62-63	33.50556771900351	36.5	32.0	39.0	23.5	40.5
64-65	33.01853196030734	36.0	31.5	39.0	22.0	40.0
66-67	32.32145396893249	35.5	31.0	39.0	19.5	40.0
68-69	31.482838237303206	35.0	30.0	38.0	15.5	40.0
70-71	30.881802607065787	34.5	30.0	37.5	5.0	40.0
72-73	29.86288992727205	34.0	29.0	37.0	2.0	39.0
74-75	29.0168757603917	34.0	29.0	36.0	2.0	39.0
76	25.448460251271676	30.0	23.0	34.0	2.0	36.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2304	1	0.11909889335552393
2304	2	0.16916532589237931
2304	3	0.14789166579909363
2304	4	0.13935435140871988
2304	5	0.0319524261630022
2304	6	0.15814393615430333
2304	7	0.08688617801091425
2304	8	0.17823835968229673
2304	9	0.23251485197773292
2304	10-11	0.12331862030966079
2304	12-13	0.14259650682924274
2304	14-15	0.03837550957755553
2304	16-17	-0.05184447377748569
2304	18-19	-0.13444283875040242
2304	20-21	-0.0688899409094077
2304	22-23	-0.05913376416707905
2304	24-25	-0.15566302375641783
2304	26-27	-0.1507817808308758
2304	28-29	-0.28056970611330456
2304	30-31	-0.33293473723177414
2304	32-33	-0.2420020244303558
2304	34-35	-0.4634696144164252
2304	36-37	-0.5305900771496255
2304	38-39	-0.4430750417732341
2304	40-41	-0.6399900162121526
2304	42-43	-0.6234310167297679
2304	44-45	-0.7547659772564046
2304	46-47	-0.8247418388150649
2304	48-49	-0.9668317735802887
2304	50-51	-0.989395443936651
2304	52-53	-0.9466588803624703
2304	54-55	-0.9437391327125155
2304	56-57	-1.2037701792758355
2304	58-59	-1.5887383798336785
2304	60-61	-1.7269725578926227
2304	62-63	-1.743760255546654
2304	64-65	-1.799120451853355
2304	66-67	-1.9975092942822954
2304	68-69	-2.0141849309486552
2304	70-71	-2.383455695645548
2304	72-73	-2.9476444340906234
2304	74-75	-2.8848150351892095
2304	76	-2.7398160824411377
2305	1	0.08484242581337753
2305	2	0.20881461652656697
2305	3	0.14277042807082552
2305	4	0.15513179579011904
2305	5	0.10963464879090878
2305	6	0.12635178319077767
2305	7	0.14618383930888967
2305	8	0.13314012109704265
2305	9	0.1823168560609787
2305	10-11	0.08350164759011136
2305	12-13	0.15636129936100218
2305	14-15	-0.028712803172531665
2305	16-17	-0.21011239319719976
2305	18-19	-0.27233455417727725
2305	20-21	-0.15403490014686838
2305	22-23	-0.20216350257265248
2305	24-25	-0.18281648644116189
2305	26-27	-0.228055732101744
2305	28-29	-0.3166522350932226
2305	30-31	-0.276790119837905
2305	32-33	-0.4263955287424679
2305	34-35	-0.37405286652838754
2305	36-37	-0.4451097435104572
2305	38-39	-0.32252529100067306
2305	40-41	-0.7233374494052995
2305	42-43	-0.9781325594726482
2305	44-45	-1.417022271479773
2305	46-47	-1.1811648086401618
2305	48-49	-1.061900732833145
2305	50-51	-1.2384532939272432
2305	52-53	-1.3851827427845151
2305	54-55	-1.4761319931388925
2305	56-57	-1.5933944951925412
2305	58-59	-1.8109814078570778
2305	60-61	-1.878155860170331
2305	62-63	-1.865641587760603
2305	64-65	-1.8532268440211865
2305	66-67	-2.0969242563076165
2305	68-69	-2.1138294617182076
2305	70-71	-2.0525835358802276
2305	72-73	-2.541459922276342
2305	74-75	-2.8237956609259918
2305	76	-2.7129323784504322
2306	1	0.0660216154933444
2306	2	0.09244861045996089
2306	3	0.04790834230117014
2306	4	0.18250743717948836
2306	5	0.07434022062660262
2306	6	0.16230978745841185
2306	7	0.08140984032672804
2306	8	0.07851706554313864
2306	9	0.0932667659047226
2306	10-11	0.08608132843131955
2306	12-13	0.1002716306265512
2306	14-15	0.0768818831451199
2306	16-17	-0.05905955252011097
2306	18-19	-0.1002809551584889
2306	20-21	-0.1053233486910301
2306	22-23	-0.14623051094734052
2306	24-25	0.0736070665526114
2306	26-27	-0.008299440468576336
2306	28-29	-0.07469357816236055
2306	30-31	-0.011722032431443097
2306	32-33	-0.18404265831019728
2306	34-35	-0.2163734231166785
2306	36-37	-0.25143565224288267
2306	38-39	-0.05793131277312824
2306	40-41	-0.5515646853136502
2306	42-43	-0.6285228364450077
2306	44-45	-0.7946274200584327
2306	46-47	-0.7447705498364314
2306	48-49	-0.6618057479506163
2306	50-51	-0.7842489434909226
2306	52-53	-1.1010443594686805
2306	54-55	-1.0608594271181104
2306	56-57	-1.0575137257693896
2306	58-59	-1.194694421099129
2306	60-61	-1.291237334978966
2306	62-63	-1.4573726383755812
2306	64-65	-1.4734288214049407
2306	66-67	-1.4471557168688776
2306	68-69	-1.5547673971091633
2306	70-71	-1.5823457301788473
2306	72-73	-2.1442077287976318
2306	74-75	-2.2092791397981983
2306	76	-2.185774753507811
2307	1	0.1198849946851368
2307	2	0.1686286661259686
2307	3	0.15460510071966382
2307	4	0.17966188508535907
2307	5	0.12359202938410618
2307	6	0.1820262801488468
2307	7	0.13372205296852968
2307	8	0.09522347676876564
2307	9	0.15958990879246215
2307	10-11	0.1353076835118756
2307	12-13	0.15795238122304056
2307	14-15	0.11077937835734986
2307	16-17	-0.053490408815996204
2307	18-19	-0.028112635841239353
2307	20-21	-0.047321932088436824
2307	22-23	-0.014020567663948214
2307	24-25	0.15835358621632167
2307	26-27	0.08837473119186967
2307	28-29	0.014948230423051712
2307	30-31	6.995706416219605E-4
2307	32-33	-0.0032544751869565403
2307	34-35	0.04792806509379943
2307	36-37	0.0513243738797442
2307	38-39	0.22993458774441677
2307	40-41	-0.08024115457632774
2307	42-43	-0.23004143899994034
2307	44-45	-0.23834575063933272
2307	46-47	-0.27995395726507866
2307	48-49	-0.21520199943176266
2307	50-51	-0.24983151481832522
2307	52-53	-0.373197428380621
2307	54-55	-0.45300626101098374
2307	56-57	-0.43672101451269896
2307	58-59	-0.5239477408360784
2307	60-61	-0.5950918316402749
2307	62-63	-0.6616479042170411
2307	64-65	-0.683274208283386
2307	66-67	-0.6284310165538152
2307	68-69	-0.666437443782133
2307	70-71	-0.617092128055063
2307	72-73	-0.9883262045094341
2307	74-75	-1.1604466833437677
2307	76	-1.2235434141120152
2308	1	0.0887525329730714
2308	2	0.08923464256007207
2308	3	0.09051927762383372
2308	4	0.14162915976842072
2308	5	0.030257638678442333
2308	6	0.10919549395956807
2308	7	0.10642315850470396
2308	8	0.09736083588853717
2308	9	0.08599425888054668
2308	10-11	0.0923233764064264
2308	12-13	0.045367549449615296
2308	14-15	0.05360174057226175
2308	16-17	-0.0328711479847712
2308	18-19	-0.07268986767032004
2308	20-21	-0.15216920524194677
2308	22-23	-0.1749802080513163
2308	24-25	-0.07025156346845307
2308	26-27	-0.06962078951102768
2308	28-29	-0.072496021453901
2308	30-31	-0.05918020984761796
2308	32-33	-0.20599363167255547
2308	34-35	-0.09182071273039583
2308	36-37	-0.11721655467462
2308	38-39	0.12993832580941245
2308	40-41	-0.03714640181880213
2308	42-43	-0.1924804722779001
2308	44-45	-0.2509595519918335
2308	46-47	-0.2748501510929202
2308	48-49	-0.297359945370566
2308	50-51	-0.2252695712574777
2308	52-53	-0.27927130845704085
2308	54-55	-0.3860553166924703
2308	56-57	-0.28574423806158933
2308	58-59	-0.45039769062423574
2308	60-61	-0.6043730388568136
2308	62-63	-0.5620627224895145
2308	64-65	-0.5627047705283417
2308	66-67	-0.584718473003516
2308	68-69	-0.7461254704852855
2308	70-71	-0.7811764820381093
2308	72-73	-0.7850596754804009
2308	74-75	-0.8409706381185096
2308	76	-0.9787191065572749
2309	1	0.06558920237325694
2309	2	0.08080017290345154
2309	3	0.03679341239892864
2309	4	0.11512933697521532
2309	5	0.040054944975452145
2309	6	0.13109221382737957
2309	7	0.06261174177649309
2309	8	0.04244870833519343
2309	9	0.01932342907749529
2309	10-11	0.03226669983275343
2309	12-13	0.038327845087039236
2309	14-15	-0.06981408751109086
2309	16-17	-0.09194183607897344
2309	18-19	-0.08917139332552892
2309	20-21	-0.039128238271032956
2309	22-23	-0.11490632766082598
2309	24-25	0.09090603013534349
2309	26-27	-0.054642653436729915
2309	28-29	-0.04546633047688431
2309	30-31	-0.016098741571639152
2309	32-33	-0.09866671564746099
2309	34-35	0.012682240906713105
2309	36-37	-0.025871632383221765
2309	38-39	0.16298224686394036
2309	40-41	0.01935274334842063
2309	42-43	-0.11073490381982509
2309	44-45	-0.1629949353465534
2309	46-47	-0.16706661154972835
2309	48-49	-0.14735160890508325
2309	50-51	0.0027044656708312687
2309	52-53	-0.1671404216073782
2309	54-55	-0.10904524768805857
2309	56-57	-0.1899196005370385
2309	58-59	-0.107456755014951
2309	60-61	-0.22817339163492534
2309	62-63	-0.36207704806369634
2309	64-65	-0.30722925309522253
2309	66-67	-0.2277405356816189
2309	68-69	-0.2968601914285536
2309	70-71	-0.2304081821113826
2309	72-73	-0.5457183718132868
2309	74-75	-0.5537721427854159
2309	76	-0.6761074298602381
2310	1	-0.030512055227703172
2310	2	0.009440914481956497
2310	3	0.012490972973296266
2310	4	0.06427611776309305
2310	5	-0.058440790523405894
2310	6	0.042031416408057964
2310	7	0.02061309689742785
2310	8	0.024477522334535706
2310	9	-0.06506304028392407
2310	10-11	-0.04614762952064666
2310	12-13	-0.0469518281255219
2310	14-15	-0.061166949951520166
2310	16-17	-0.07658815949101694
2310	18-19	-0.11979933340798254
2310	20-21	-0.14243702076961284
2310	22-23	-0.15877825514616717
2310	24-25	0.009607969569884744
2310	26-27	-0.08685216524012418
2310	28-29	-0.007774212350923904
2310	30-31	0.06427435022726513
2310	32-33	-0.10551021715918552
2310	34-35	0.06435885355557502
2310	36-37	0.005934635546630318
2310	38-39	0.2910033383640851
2310	40-41	0.09343856424571584
2310	42-43	-0.02083268025494789
2310	44-45	0.008680710688949489
2310	46-47	-0.007370952270854048
2310	48-49	0.1131006189806385
2310	50-51	0.16457335925947802
2310	52-53	0.03006842893078243
2310	54-55	0.01578980709578559
2310	56-57	0.027720825125655324
2310	58-59	0.056368660891308764
2310	60-61	0.03624452337581374
2310	62-63	-0.02528744325278609
2310	64-65	-0.09172891041524878
2310	66-67	0.11588243227319595
2310	68-69	0.06106698389789855
2310	70-71	0.09572869636198789
2310	72-73	-0.2567979532918052
2310	74-75	-0.2422561523921125
2310	76	-0.33117040499709205
2311	1	-0.059841636314583724
2311	2	-0.048905123953609575
2311	3	-0.035615952355946945
2311	4	0.0025582626956079935
2311	5	-0.015961575382043236
2311	6	0.033503952408032944
2311	7	-0.028978044392445668
2311	8	-0.04806449933619916
2311	9	-0.03858883328765472
2311	10-11	-0.06635882033252472
2311	12-13	-0.0792954158937178
2311	14-15	-0.11146367423823023
2311	16-17	-0.07208606283733587
2311	18-19	-0.12220371998495949
2311	20-21	-0.13935054366967847
2311	22-23	-0.1328028759046731
2311	24-25	0.012314593492448012
2311	26-27	2.039801002808872E-4
2311	28-29	-1.9979132336800376E-4
2311	30-31	0.11768489839065666
2311	32-33	0.02395879193540651
2311	34-35	0.07933186860577024
2311	36-37	0.042646389080566394
2311	38-39	0.30755580690925655
2311	40-41	0.0893956969976415
2311	42-43	0.03617236989039441
2311	44-45	0.08258611129033255
2311	46-47	0.09369435271668891
2311	48-49	0.19191251985920843
2311	50-51	0.1761369183546435
2311	52-53	0.1256231977411275
2311	54-55	0.16725961845813941
2311	56-57	0.21778209539073146
2311	58-59	0.05950743125494995
2311	60-61	0.0872853156364215
2311	62-63	0.07729179107766271
2311	64-65	0.05381092324115144
2311	66-67	0.2836453530272962
2311	68-69	0.23725446225221347
2311	70-71	0.2592989529796874
2311	72-73	-0.04653369017395548
2311	74-75	0.06673075119610061
2311	76	-8.052953876678259E-4
2312	1	-0.11325776032301249
2312	2	-0.11260781746159054
2312	3	-0.10766782420803622
2312	4	-0.029387907371251742
2312	5	-0.12526222772481788
2312	6	-0.048098275445973115
2312	7	-0.10086198470254715
2312	8	-0.08660028639709338
2312	9	-0.1762358369537722
2312	10-11	-0.137514395066205
2312	12-13	-0.20455895853313422
2312	14-15	-0.17854046760530906
2312	16-17	-0.15118915911354947
2312	18-19	-0.04888192677780978
2312	20-21	-0.06468485930447088
2312	22-23	-0.025189794172867153
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1307	3	0.1723743703297842
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1307	10-11	0.14936022028760476
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1311	1	-0.28912189221861695
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1311	10-11	-0.4198189689537912
1311	12-13	-0.3955519806688983
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1311	16-17	-0.42989453119201926
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2301	3	-0.34183523561256735
2301	4	-3.710922213591587
2301	5	-1.2792119798562993
2301	6	-0.5613868452366475
2301	7	-0.3863130806100372
2301	8	-0.29950587461839007
2301	9	-0.2768015887569035
2301	10-11	-0.2853838512271665
2301	12-13	-0.19728587940651465
2301	14-15	-0.3139601335496778
2301	16-17	-0.5078733886423095
2301	18-19	-0.06149244385619568
2301	20-21	0.11982166190381349
2301	22-23	0.17233590839870772
2301	24-25	0.11119749136199175
2301	26-27	0.0961137949153823
2301	28-29	0.019732094576419
2301	30-31	0.25859212330997394
2301	32-33	0.2562153359923869
2301	34-35	0.2552168020748695
2301	36-37	0.36899366151856583
2301	38-39	0.6668903515119879
2301	40-41	0.5630569762083653
2301	42-43	0.4924812631815385
2301	44-45	0.4934395473367985
2301	46-47	0.6033066172689487
2301	48-49	0.45153642485137624
2301	50-51	0.5686516508757364
2301	52-53	0.6480027410054063
2301	54-55	0.7515937283806764
2301	56-57	0.7956619998109034
2301	58-59	0.7558310229912308
2301	60-61	0.8680703566345187
2301	62-63	0.9660981168387579
2301	64-65	0.9473251744349582
2301	66-67	1.1828517234315754
2301	68-69	1.2324880408282546
2301	70-71	1.0670617735829317
2301	72-73	1.3408441478877116
2301	74-75	1.55619207568947
2301	76	1.7688088489363558
2302	1	-0.2861867293414271
2302	2	-0.33943202570783626
2302	3	-0.30567959168936554
2302	4	-0.20727346625843523
2302	5	-0.1867159447875295
2302	6	-0.20284834122027462
2302	7	-0.2877687613139912
2302	8	-0.3239647520300153
2302	9	-0.8645266997439407
2302	10-11	-0.5132700531109862
2302	12-13	-0.3799878586604848
2302	14-15	-0.36285167548421526
2302	16-17	-0.2625766795112554
2302	18-19	-0.13990487448327116
2302	20-21	-0.1023578405119352
2302	22-23	-0.009688707401927843
2302	24-25	0.04463778281551356
2302	26-27	-0.05912267649973302
2302	28-29	-0.16955811084807948
2302	30-31	0.1080569556516977
2302	32-33	0.03656986208014956
2302	34-35	0.029898691781077957
2302	36-37	0.144651645127162
2302	38-39	0.2780343394262559
2302	40-41	0.3274154953403041
2302	42-43	0.3508732753822912
2302	44-45	0.24632365913089416
2302	46-47	0.26588951955103823
2302	48-49	0.48092995939310157
2302	50-51	0.5730997091520891
2302	52-53	0.5515439450595991
2302	54-55	0.5612518114415508
2302	56-57	0.5662686755385238
2302	58-59	0.6127015305342312
2302	60-61	0.6909990341330712
2302	62-63	0.6352395137125413
2302	64-65	0.7316570198892123
2302	66-67	0.8389322819807887
2302	68-69	0.980140458423957
2302	70-71	0.9379017324424979
2302	72-73	1.3308528540647124
2302	74-75	1.4560782260187004
2302	76	1.4925892198102204
2303	1	-0.4687620417461815
2303	2	-0.530378364395812
2303	3	-0.6083881971719585
2303	4	-0.3536079346268295
2303	5	-0.4724369619678015
2303	6	-0.5281157062437387
2303	7	-0.5058300774007947
2303	8	-0.5299322538773481
2303	9	-0.8222021425248158
2303	10-11	-0.7195399355041587
2303	12-13	-0.6157892967790843
2303	14-15	-0.5924354587660616
2303	16-17	-0.5921279743427803
2303	18-19	-0.5959145706082225
2303	20-21	-0.36013724888766774
2303	22-23	-0.31758273258162006
2303	24-25	-0.4369427649383013
2303	26-27	-0.46477228558956796
2303	28-29	-0.47756891910476895
2303	30-31	-0.3903645975467427
2303	32-33	-0.5775186297322534
2303	34-35	-0.4515507629988136
2303	36-37	-0.5812280959484397
2303	38-39	-0.10885678100769525
2303	40-41	-0.30876660744131357
2303	42-43	-0.38319642392863074
2303	44-45	-0.15182792658388422
2303	46-47	-0.07118633353426418
2303	48-49	-0.039418384743989066
2303	50-51	-0.16901307642830687
2303	52-53	-0.14604003978850955
2303	54-55	-0.015070483638240262
2303	56-57	0.07051006433757578
2303	58-59	-0.277011932002587
2303	60-61	-0.24524833215041042
2303	62-63	-0.10422639095462927
2303	64-65	-0.2639888974646496
2303	66-67	-0.18713172130969724
2303	68-69	0.042625154891386074
2303	70-71	0.04212324241489185
2303	72-73	0.21769858650493745
2303	74-75	0.18167246765018064
2303	76	0.137052470872284
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12408.0
3	1141.0
4	3382.0
5	6064.0
6	9708.0
7	15372.0
8	25108.0
9	46038.0
10	82941.0
11	127855.0
12	171321.0
13	222506.0
14	290604.0
15	369980.0
16	454331.0
17	535408.0
18	623175.0
19	716146.0
20	820473.0
21	944485.0
22	1100587.0
23	1301555.0
24	1552573.0
25	1877961.0
26	2278566.0
27	2789904.0
28	3418759.0
29	4178111.0
30	5080620.0
31	6182689.0
32	7574449.0
33	9440899.0
34	1.2029808E7
35	1.5766716E7
36	2.1395716E7
37	2.9705759E7
38	3.8659466E7
39	3.0651792E7
40	534004.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.20805229863365	16.24691119765445	14.609921473524693	41.935115030187205
2	20.384359552921424	24.178889982182113	36.754278135345494	18.68247232955097
3	22.450833185819707	26.40033267929821	27.042841340313288	24.10599279456879
4	23.483642504979393	32.730838427653	22.09049346566873	21.69502560169888
5	24.010896505733033	35.606724790518214	23.726350431282086	16.656028272466674
6	19.446309965284296	38.50828797724638	24.583163804603796	17.462238252865518
7	15.5514512107013	21.26874505157703	43.99434811365147	19.185455624070205
8	17.563424640536905	26.596266596775557	32.41143087819911	23.428877884488422
9	20.26202300734961	23.73218182156493	33.566776010831525	22.439019160253928
10-11	21.562940208771835	35.086161390952505	23.48506813836012	19.865830261915544
12-13	20.353007435813186	28.13255106200307	29.401796714240014	22.11264478794373
14-15	20.04317647776449	29.078800405288874	29.57228424539238	21.305738871554254
16-17	21.42423933964045	28.77466350723822	28.388780546390475	21.41231660673086
18-19	20.9109411727597	28.721615318491622	29.021359774143452	21.346083734605223
20-21	21.394006713911924	28.926520721270226	28.948171576506493	20.731300988311354
22-23	20.690962363267857	30.372293180055586	28.108809868105787	20.827934588570766
24-25	20.433747276968102	29.51192691204775	28.981606717427276	21.072719093556874
26-27	20.2189229087319	28.95527615695211	28.647750295300888	22.1780506390151
28-29	20.936831131700924	29.535209863222846	28.411301228035583	21.116657777040647
30-31	20.638866367171847	28.844632806237673	29.237802407897355	21.27869841869312
32-33	20.491951697878694	29.871633428715022	28.415715543821758	21.220699329584527
34-35	21.655220851109565	29.47196504658433	28.20166538468537	20.671148717620735
36-37	20.81895870108111	28.984753199971813	28.73592226723233	21.460365831714746
38-39	21.064474242481467	29.346035688281862	28.874831277061787	20.714658792174887
40-41	20.829484795817894	29.25997741381946	29.155901988014634	20.754635802348016
42-43	20.6591468995296	28.821291365332417	29.304745729907822	21.214816005230162
44-45	21.122486937690894	28.840119967461398	28.627375043001095	21.410018051846613
46-47	20.92584528478566	29.106408269069394	29.039922737744238	20.92782370840071
48-49	21.228561899920035	28.88459650372982	29.170047604712206	20.716793991637935
50-51	20.872809902096282	28.739661308914698	28.435143894583543	21.952384894405476
52-53	21.40433387748638	28.644137795320162	28.94536658642438	21.006161740769077
54-55	20.839961544404158	28.548540515429377	29.234209716801026	21.377288223365447
56-57	20.819716366612326	28.708331250955727	29.299311632508335	21.17264074992361
58-59	21.373445411650454	28.63021266500501	29.15340507625203	20.842936847092496
60-61	20.86388725829464	28.641559849819576	29.116205669737518	21.378347222148275
62-63	21.41204996511698	28.582539007568354	29.325910092338553	20.67950093497611
64-65	21.224577494597145	29.43899197331569	28.737714647913883	20.598715884173284
66-67	20.701683717645757	30.08235668445397	28.647431291539316	20.568528306360953
68-69	20.68754556205541	30.3169973679499	28.31336372649335	20.68209334350134
70-71	20.949409393249834	29.890021252907612	28.264992484019025	20.89557686982353
72-73	20.980719127201763	29.252108381897557	28.730028907470285	21.0371435834304
74-75	21.385245493023376	29.498247199803302	28.509087734935974	20.607419572237347
76	21.078992775961677	29.700043851099693	28.3093306523167	20.911632720621927
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	269.0
1	220.0
2	194.0
3	260.5
4	304.0
5	416.5
6	723.5
7	1183.0
8	1448.0
9	1919.0
10	3308.5
11	5704.0
12	7181.0
13	10646.5
14	21175.0
15	40056.5
16	74817.5
17	97760.0
18	154657.0
19	288008.5
20	486898.5
21	609334.0
22	846689.0
23	1281982.5
24	1752293.5
25	2024666.0
26	2382536.5
27	3083300.0
28	3780579.5
29	4134966.0
30	4509449.5
31	5385126.5
32	6227090.0
33	6567860.0
34	6949204.0
35	7836923.5
36	8585351.5
37	8827404.0
38	9190193.0
39	1.00288735E7
40	1.08985665E7
41	1.1696175E7
42	1.2099982E7
43	1.24906495E7
44	1.29436125E7
45	1.2554614E7
46	1.210332E7
47	1.16450795E7
48	1.0461945E7
49	9006952.0
50	8276853.0
51	7951292.5
52	6801443.0
53	5357925.0
54	4738696.0
55	4198932.5
56	3212529.5
57	2420645.5
58	2075401.0
59	1810917.0
60	1320098.0
61	914620.0
62	735477.0
63	633862.5
64	468669.0
65	355616.5
66	267474.5
67	228806.0
68	198452.0
69	139540.5
70	96267.5
71	81552.0
72	73061.5
73	56761.0
74	39022.5
75	29094.0
76	22424.0
77	12225.5
78	6913.5
79	5130.0
80	4274.5
81	2826.5
82	1822.0
83	1410.0
84	1146.5
85	715.0
86	464.0
87	381.0
88	316.5
89	204.5
90	131.0
91	92.0
92	79.0
93	73.0
94	47.0
95	30.5
96	34.0
97	22.5
98	7.5
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11001431951839613
2	0.007154784033582759
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	5.957759460548886E-4
14-15	2.54479662970418E-4
16-17	0.0
18-19	0.0
20-21	8.462754774441466E-4
22-23	7.097072125655938E-4
24-25	0.0
26-27	0.0
28-29	0.001423145798488525
30-31	5.099543588361259E-4
32-33	1.661704935134303E-4
34-35	0.009507041798048323
36-37	0.013724737482958816
38-39	0.012641892934659473
40-41	0.010478442662075187
42-43	0.0051393449041728595
44-45	0.0010176698936578495
46-47	0.0025000201494161296
48-49	0.0037627168935391417
50-51	9.099575827427067E-4
52-53	0.0015674255683055755
54-55	0.0011962782983624047
56-57	0.003072164064207881
58-59	0.01257796207113709
60-61	0.01597525313388098
62-63	0.012203332186060404
64-65	0.019372295438401046
66-67	0.01857701539684051
68-69	0.1493855323610071
70-71	0.0
72-73	0.012558558929678937
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	2.0099838E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.65763070300249
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.9596544343731	27.1118569917693
2	12.108113482155845	8.87709505077829
3	4.747093067119438	5.220515537017432
4	2.5185281554164027	3.692931001454742
5	1.4898187432184185	2.730661265165604
6	1.1704402778292033	2.574334047874954
7	0.6328949329641201	1.6240300108480161
8	0.5291816401743533	1.5518836112256469
9	0.29536342040387836	0.9744590869506939
>10	2.1968655539722612	14.645174224357172
>50	0.18669473512741885	4.794517912151249
>100	0.14393041251476887	10.433780236363388
>500	0.01305379743315653	3.29706680150662
>1k	0.007162276137307781	4.81331699773815
>5k	6.538532741215225E-4	1.6920874790928648
>10k+	5.512178863760459E-4	5.966289745705778
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC	1047892	0.5213435053556154	TruSeq Adapter, Index 9 (100% over 50bp)
CCCTGATTCAACATCGAGGTGCCAATCCCACTAGCCAATACGTTCTCTAC	283529	0.14106034088433947	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.30897860967834667	8.955296057610017E-6	2.9850986858700055E-6
2	0.30932139851077406	9.950328952900018E-6	9.452812505255017E-6
3	0.30969055571492665	1.044784540054502E-5	1.094536184819002E-5
4	0.3109049933636281	1.094536184819002E-5	1.641804277228503E-5
5	0.31134629045268924	1.1940394743480022E-5	1.691555921993003E-5
6	0.3120418184464969	1.7413075667575034E-5	1.7413075667575034E-5
7	0.3124731652066052	1.8905625010510035E-5	1.7413075667575034E-5
8	0.3139264107501762	2.139320724873504E-5	1.9403141458155035E-5
9	0.31463139155648917	2.3383273039315044E-5	2.4378305934605045E-5
10	0.31605727369543973	2.636837172518505E-5	2.587085527754005E-5
11	0.31712195889340006	3.1343536201635056E-5	2.8855953963410054E-5
12	0.31810853400908007	3.233856909692506E-5	3.233856909692506E-5
13	0.31955879445396523	3.4328634887505064E-5	3.582118423044007E-5
14	0.3207513413789703	3.681621712573007E-5	3.880628291631007E-5
15	0.3220339387809991	3.830876646866507E-5	4.079634870689008E-5
16	0.3232453913310147	3.980131581160007E-5	4.179138160218008E-5
17	0.3247100797528816	4.278641449747008E-5	4.477648028805008E-5
18	0.32618969366817785	4.5273996735695084E-5	5.771190792682011E-5
19	0.327571296843288	4.676654607863009E-5	5.870694082211011E-5
20	0.3290787716796523	4.676654607863009E-5	5.920445726975511E-5
21	0.33087082592406963	4.776157897392009E-5	6.119452306033511E-5
22	0.33261959623754184	5.0249161212145094E-5	6.517465464149512E-5
23	0.33400617457712845	5.57218421362401E-5	7.064733556559013E-5
24	0.3352086718310864	6.019949016504511E-5	7.363243425146014E-5
25	0.33630818318038186	6.517465464149512E-5	7.512498359439514E-5
26	0.33743704800008834	6.766223687972012E-5	7.761256583262014E-5
27	0.3385345692835932	7.164236846088014E-5	7.960263162320014E-5
28	0.33967985214607205	7.462746714675013E-5	8.010014807084514E-5
29	0.3407947865052445	7.612001648968514E-5	8.258773030907015E-5
30	0.3421420610454671	7.711504938497514E-5	8.806041123316516E-5
31	0.3433659515066738	7.910511517555515E-5	8.855792768081017E-5
32	0.34474655964888873	8.258773030907015E-5	9.054799347139017E-5
33	0.346080401245025	8.358276320436016E-5	9.353309215726018E-5
34	0.3474470789267058	8.557282899494016E-5	9.403060860490518E-5
35	0.3487759453583656	8.955296057610016E-5	9.552315794784018E-5
36	0.35011774721766414	9.303557570961517E-5	9.751322373842018E-5
37	0.35155407720201526	9.651819084313018E-5	9.900577308135519E-5
38	0.35306403962061783	1.0099583887193519E-4	1.0149335531958018E-4
39	0.35461877851950846	1.0248838821487019E-4	1.034834211101602E-4
40	0.3560715265466319	1.059710033483852E-4	1.0646851979603019E-4
41	0.35764019590605656	1.1243871716777021E-4	1.1343375006306022E-4
42	0.3594019016471675	1.1691636519657521E-4	1.2388159546360524E-4
43	0.3611412191481344	1.1990146388244522E-4	1.2587166125418523E-4
44	0.36278451597470585	1.2139401322538022E-4	1.2786172704476524E-4
45	0.3644307978999632	1.2189152967302522E-4	1.3084682573063524E-4
46	0.3660989705489169	1.2288656256831523E-4	1.3482695731179524E-4
47	0.3679532143492898	1.2338407901596023E-4	1.4029963823589025E-4
48	0.36975919905424115	1.2736421059712024E-4	1.4477728626469526E-4
49	0.37158010925262186	1.2885675994005525E-4	1.4676735205527527E-4
50	0.3736258968853381	1.2935427638770025E-4	1.4975245074114527E-4
51	0.375709993284523	1.3184185862592524E-4	1.527375494270153E-4
52	0.37835628326954673	1.3631950665473025E-4	1.572151974558203E-4
53	0.38069212299124006	1.3830957244531026E-4	1.641804277228503E-4
54	0.38310358521297533	1.4029963823589025E-4	1.656729770657853E-4
55	0.3854986293919384	1.4129467113118026E-4	1.706481415422353E-4
56	0.3879827290150299	1.4477728626469526E-4	1.7214069088517032E-4
57	0.3905265306118388	1.4825990139821028E-4	1.7562330601868532E-4
58	0.39329570715943085	1.532350658746603E-4	1.8109598694278034E-4
59	0.39616687457879013	1.5472761521759528E-4	1.8209101983807034E-4
60	0.39935346742595634	1.562201645605303E-4	1.8408108562865033E-4
61	0.40276394267456284	1.587077467987553E-4	1.8557363497158534E-4
62	0.4066201926602592	1.621903619322703E-4	1.8855873365745534E-4
63	0.41108440774497784	1.666680099610753E-4	1.9452893102919535E-4
64	0.4179546123705077	1.741307566757503E-4	1.9701651326742037E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACACG	365355	0.0	48.6127	11
GTCACGA	196620	0.0	47.820133	29
CACGATC	201415	0.0	47.091522	31
CACACGT	377090	0.0	46.632286	12
GATCGGA	382925	0.0	46.485443	1
CACGTCT	377385	0.0	46.38316	14
AGTCACG	241160	0.0	46.270905	28
ACACGTC	381650	0.0	45.97061	13
CGGAAGA	389380	0.0	45.65039	4
TCGGAAG	390380	0.0	45.532555	3
GAGCACA	394755	0.0	45.254528	9
AGAGCAC	396395	0.0	45.12201	8
AGCACAC	398095	0.0	44.832657	10
ATCGGAA	404675	0.0	43.99815	2
ACGTCTG	398125	0.0	43.936558	15
CGTCTGA	399215	0.0	43.761192	16
TCACGAT	215985	0.0	43.39598	30
CGATCAG	217785	0.0	43.10973	33
GAACTCC	409540	0.0	42.54593	21
ACGATCA	222655	0.0	42.3939	32
>>END_MODULE