FastQCFastQC Report
Fri 2 Oct 2015
OlyOv6_combined_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameOlyOv6_combined_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200998380
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4693250.23349690679099006Illumina Single End PCR Primer 1 (100% over 50bp)
CCCTGATTCAACATCGAGGTGCCAATCCCACTAGCCAATACGTTCTCTAC3096450.1540534804310363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA932300.044.81511747
TCGCCGT940250.043.6364546
GCGTCGT996700.042.27760312
GTCGCCG991500.042.27674545
TGGTCGC963350.042.0485343
GGTCGCC1011400.041.80455844
AGCGTCG1049400.040.571311
GCCGTAT1068000.039.3894548
GTGGTCG1038400.039.02887742
CGTCGTG1119150.037.63879813
CGTGTAG1108050.037.55217716
CCGTATC1130750.037.17442349
GAGCGTC1153450.037.10828810
GTAGGGA1172400.036.26698719
TCGGTGG1199450.035.0773239
CGTATCA1208450.034.6003150
AGAGCGT1248400.034.3567169
TCGTGTA1212150.034.3355215
AGATCGG1260450.034.2435871
ATCTCGG1273450.034.2215836
Produced by FastQC (version 0.11.2)