FastQCFastQC Report
Thu 2 Jul 2015
R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937202899
Sequences flagged as poor quality0
Sequence length36-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC10478920.11181058030423356TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC2634350.092.4534931
CGATCAG2908000.082.9031633
TATGCCG4263550.082.6245246
ACGATCA3006650.080.61487632
GATCTCG2759350.078.1810839
TCGTATG4534350.078.0159243
CGTATGC4591900.077.05141444
ATGCCGT4520250.074.87366547
GTATGCC4956850.071.3326945
TGCCGTC4764050.071.0646348
GCCGTCT4940300.068.1589849
TCTCGTA4862300.068.0272241
ATCTCGT4976900.066.5245840
CCGTCTT5169650.065.66537550
CTCGTAT5681750.060.50584842
TAGCTTA1480600.059.7821134
CTGCTTG5783900.059.1774357
GCTTGAA4800600.058.92211559
CGTCTTC5881850.057.76938251
CTTCTGC6138200.057.2079154
Produced by FastQC (version 0.11.2)