FastQCFastQC Report
Thu 2 Jul 2015
R1_unpaired_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR1_unpaired_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1655750
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA18700.053.2451861
ATCGGAA22350.047.2285082
TCGGAAG19350.042.3967633
CGGAAGA18700.042.0764854
TCGCAAG5550.040.1135643
GATGGGA13650.037.7296331
GGAAGAG40150.036.535995
ATCGCAA6150.036.2120742
GATCGCA6400.035.6019821
CGCAAGA6450.034.043494
TGGGAAG15000.033.9536553
AAGAGCA54300.032.0137377
GATCGTA3750.031.6288641
GAGCACA62150.030.3255529
AGAGCAC59250.030.265678
GGGAAGA17350.029.8820784
GAAGAGC45700.029.7632276
GCAAGAG7400.029.6730465
ATCGGGA3100.029.5233172
CGTAAGA3950.029.3391744
Produced by FastQC (version 0.11.2)