FastQCFastQC Report
Fri 3 Jul 2015
R1_val_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR1_val_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195953013
Sequences flagged as poor quality0
Sequence length20-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTGATTCAACATCGAGGTGCCAATCCCACTAGCCAATACGTTCTCTAC2185860.11155021127437321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGGC1410950.023.0255370
ATTAGCC1357850.016.80688957
TCCCCGG1456250.016.72889569
TAGCCTG1407650.016.39617759
ACTAGCC1395900.015.9535230
TACGTTC1414050.015.88133239
CCCACTA1432750.015.67361627
ATCCCCG1487850.015.63841268
TTAGCCT1476550.015.56739958
CTAGCCA1493550.015.14535731
TCGAGGT1467900.015.07705414
CGAGGTG1474100.015.00625615
CACTAGC1510850.014.83256429
CTACTAG1572200.014.58881747
GAGGTGC1522500.014.58052316
GGATTAG1580150.014.56855755
ATACGTT1555100.014.46787638
CCCGGCG1088800.014.2785370
ACGGTCC456800.014.25947146
CAATACG1596650.014.10361336
Produced by FastQC (version 0.11.2)