FastQCFastQC Report
Thu 2 Jul 2015
R2_unpaired_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR2_unpaired_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83063
Sequences flagged as poor quality0
Sequence length35-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGCTCGGGGGTCGATGAAGAGCGCAGCCAGCTGCGTGAATTAATGTG1920.23114985011376907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGAC600.0119.1512870
AGGGTCG150.003312649763.26024210
GAAGACG355.9213016E-754.2230647
GCGCCGT200.00676073252.82293745
AAAAGCG253.920336E-450.6081967
ATGCCCT200.00835614950.06929437
GAACATC957.2759576E-1249.882267
AACATCG1051.0913936E-1147.8066168
AAGACGG401.4934612E-647.416628
AGGGTAG654.9294613E-1043.76918828
TGCAGGA904.1836756E-1142.3060254
ATTTAGC309.628029E-442.17349623
CGGCATA453.3456909E-642.17349212
AGGACAC954.9112714E-1141.58726557
ACATCGA1208.421921E-1041.3650269
TCGGCTC808.185452E-1139.6810762
CTCGGCT808.185452E-1139.6810761
CGGCTCG808.185452E-1139.6810763
GATCGGA907.2759576E-1238.799271
GAATTGC951.1459633E-1038.46170850
Produced by FastQC (version 0.11.2)