FastQCFastQC Report
Fri 3 Jul 2015
R2_val_2.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameR2_val_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195953013
Sequences flagged as poor quality0
Sequence length20-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTGATTCAACATCGAGGTGCCAATCCCACTAGCCAATACGTTCTCTAC2802810.1430347998782749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGGC1561700.024.94234370
ATTAGCC1432250.017.6903957
TCCCCGG1579400.017.32305369
ACTAGCC1452400.017.15756830
TAGCCTG1478850.017.15034159
TACGTTC1467050.016.99236539
CCCACTA1488550.016.79109227
TTAGCCT1538000.016.52419758
ATCCCCG1599900.016.2204368
CTAGCCA1552350.016.1577231
TCGAGGT1510400.016.08957714
CACTAGC1568800.015.9924229
CGAGGTG1526100.015.94875815
ATACGTT1616950.015.55436538
GAGGTGC1574650.015.47554316
CCCGGCG1158850.015.43190970
CTACTAG1653800.015.39834447
GGATTAG1655050.015.34563655
AATACGT1662750.015.118854537
CAATACG1667300.015.02120136
Produced by FastQC (version 0.11.2)