FastQCFastQC Report
Wed 27 May 2015
filtered_106A_Female_Mix_GATCAG_L004_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefiltered_106A_Female_Mix_GATCAG_L004_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39823239
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC10454402.6252008280893477TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1614130.40532363527738163TruSeq Adapter, Index 9 (100% over 49bp)
CCCTGATTCAACATCGAGGTGCCAATCCCACTAGCCAATACGTTCTCTAC600060.15068086249840199No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC443740.11142740046835467TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1714250.057.07986511
CACACGT1731200.056.07237612
TATGCCG1603100.055.88456746
GATCGGA1757650.055.883791
CACGTCT1735750.055.65877514
ACACGTC1745200.055.49627313
AGAGCAC1774300.055.398458
TCGGAAG1768000.055.385273
GTCACGA1685250.055.27295729
CACGATC1705400.055.20088231
CGGAAGA1773500.055.1878624
CGTATGC1629200.055.1659344
AGCACAC1780100.055.12164710
GAGCACA1787650.054.9221159
AGTCACG1720500.054.56733728
ATCGGAA1801000.054.4902042
TGCCGTC1582700.054.31257248
CGATCAG1715400.054.27483433
ATGCCGT1585750.054.26767347
CGTCTGA1776750.054.20853816
Produced by FastQC (version 0.11.2)