FastQCFastQC Report
Wed 27 May 2015
filtered_106A_Female_Mix_GATCAG_L007_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefiltered_106A_Female_Mix_GATCAG_L007_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39233599
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT14281043.640002539659948TruSeq Adapter, Index 9 (100% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA2224120.5668916583462047TruSeq Adapter, Index 7 (97% over 35bp)
CCCTGATTCAACATCGAGGTGCCAATCCCACTAGCC607760.1549080419565893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAT1609750.028.05888230
AGATCGG271850.025.869581
GCACACG1814450.025.4381111
GATCGGA1795250.025.411821
CGGAAGA1837250.025.2737314
TCGGAAG1809500.025.2351933
CACACGT1831450.025.18069512
AGAGCAC1841000.025.1666568
CACGTCT1833700.025.13098514
ACACGTC1837450.025.12703913
AGCACAC1841650.025.11863110
ATCGGAA1820300.025.076362
GAGCACA1846850.025.0633879
GAAGAGC1858400.024.943946
CGTCTGA1852000.024.83973716
ACGTCTG1857000.024.7857815
AAGAGCA1879850.024.6824537
GTCACGA1833250.024.66345429
GGAAGAG1897000.024.5274945
AGTCACG1850750.024.50885628
Produced by FastQC (version 0.11.2)