BLASTN 2.5.0+ Reference: Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000), "A greedy algorithm for aligning DNA sequences", J Comput Biol 2000; 7(1-2):203-14. Database: All GenBank+EMBL+DDBJ+PDB sequences (but no EST, STS, GSS,environmental samples or phase 0, 1 or 2 HTGS sequences) 16,028,664 sequences; 41,069,666,496 total letters Query= scaffold103946-20422 Length=698 ***** No hits found ***** Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 26959579188360 Query= scaffold106982-14135 Length=344 ***** No hits found ***** Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 12653705765376 Query= scaffold107590-8577 Length=714 ***** No hits found ***** Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 27608230717704 Query= scaffold112102-14447 Length=1474 ***** No hits found ***** Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 58355556364800 Query= scaffold11815-14456 Length=992 ***** No hits found ***** Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 38878551040056 Query= scaffold11917-28699 Length=1218 ***** No hits found ***** Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 47981235233280 Query= scaffold1242-12404 Length=882 ***** No hits found ***** Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 34419071775816 Query= scaffold126195-1416 Length=1002 ***** No hits found ***** Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 39283958245896 Query= scaffold128214-5365 Length=771 ***** No hits found ***** Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 29919051790992 Query= scaffold129719-115XX Length=569 ***** No hits found ***** Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 21729826233024 Query= scaffold130369-15322 Length=1099 ***** No hits found ***** Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 43216408142544 Query= scaffold13704-29380 Length=1035 Score E Sequences producing significant alignments: (Bits) Value NG_007520.1 Oikopleura dioica BAC clone OIKO005, complete sequence 56.5 4e-04 >NG_007520.1 Oikopleura dioica BAC clone OIKO005, complete sequence AY449461.1 Oikopleura dioica clone BACOIKO005 4xj24, complete sequence Length=95978 Score = 56.5 bits (30), Expect = 4e-04 Identities = 32/33 (97%), Gaps = 0/33 (0%) Strand=Plus/Plus Query 257 CAACTACAACAGCAACAACAACTACAACATCAA 289 ||||||||||||||||||||||||||||| ||| Sbjct 20742 CAACTACAACAGCAACAACAACTACAACAACAA 20774 Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 40621802025168 Query= scaffold147711-174 Length=445 ***** No hits found ***** Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 16749937439424 Query= scaffold16116-7076 Length=1130 ***** No hits found ***** Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 44473170480648 Query= scaffold176442-9109 Length=919 ***** No hits found ***** Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 35919078437424 Query= scaffold180853-5067 Length=1013 ***** No hits found ***** Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 39729906172320 Query= scaffold193328-3840 Length=1039 ***** No hits found ***** Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 40783964907504 Query= scaffold20564-10594 Length=1135 Score E Sequences producing significant alignments: (Bits) Value AC150407.2 Branchiostoma floridae clone CH302-54J24, complete se... 104 2e-18 AC150392.2 Branchiostoma floridae clone CH302-12P3, complete seq... 99.0 7e-17 >AC150407.2 Branchiostoma floridae clone CH302-54J24, complete sequence Length=175896 Score = 104 bits (56), Expect = 2e-18 Identities = 79/90 (88%), Gaps = 1/90 (1%) Strand=Plus/Minus Query 468 TGGTCTAGT-GGTAGAGTGTCCGCCTCGCATGTGGAAGGTTGTGGGTTCAATCCCCGGCC 526 ||| ||||| |||||||||| ||||| |||| || |||| ||| |||| |||||||||| Sbjct 121746 TGGCCTAGTGGGTAGAGTGTTCGCCTTGCATTCGGTAGGTCGTGAGTTCGATCCCCGGCC 121687 Query 527 GGGTCATACCAAAGACTTTAAAAATGGTAC 556 | |||||||||||||||||||||||||||| Sbjct 121686 GAGTCATACCAAAGACTTTAAAAATGGTAC 121657 >AC150392.2 Branchiostoma floridae clone CH302-12P3, complete sequence Length=180902 Score = 99.0 bits (53), Expect = 7e-17 Identities = 78/90 (87%), Gaps = 1/90 (1%) Strand=Plus/Plus Query 468 TGGTCTAGT-GGTAGAGTGTCCGCCTCGCATGTGGAAGGTTGTGGGTTCAATCCCCGGCC 526 ||| | ||| |||||||||| ||||| |||| || |||| ||| |||| |||||||||| Sbjct 59659 TGGCCAAGTGGGTAGAGTGTTCGCCTTGCATTCGGTAGGTCGTGAGTTCGATCCCCGGCC 59718 Query 527 GGGTCATACCAAAGACTTTAAAAATGGTAC 556 | |||||||||||||||||||||||||||| Sbjct 59719 GAGTCATACCAAAGACTTTAAAAATGGTAC 59748 Lambda K H 1.33 0.621 1.12 Gapped Lambda K H 1.28 0.460 0.850 Effective search space used: 44675874083568 Database: All GenBank+EMBL+DDBJ+PDB sequences (but no EST, STS, GSS,environmental samples or phase 0, 1 or 2 HTGS sequences) Posted date: May 12, 2012 4:43 PM Number of letters in database: 41,069,666,496 Number of sequences in database: 16,028,664 Matrix: blastn matrix 1 -2 Gap Penalties: Existence: 0, Extension: 2.5