SampleNames: Actin_HC_1 Actin_NC_1 Actin_SC_1 Actin_HT_1 Actin_NT_1 Actin_ST_1 Actin_NTC_1 Actin_HC_2 Actin_NC_2 Actin_SC_2 Actin_HT_2 Actin_NT_2 Actin_ST_2 Actin_NTC_2 Actin_HC_3 Actin_NC_3 Actin_SC_3 Actin_HT_3 Actin_NT_3 Actin_ST_3 Actin_NTC_3 Actin_HC_4 Actin_NC_4 Actin_SC_4 Actin_HT_4 Actin_NT_4 Actin_ST_4 Actin_NTC_4 Actin_HC_5 Actin_NC_5 Actin_SC_5 Actin_HT_5 Actin_NT_5 Actin_ST_5 Actin_HC_6 Actin_NC_6 Actin_SC_6 Actin_HT_6 Actin_NT_6 Actin_ST_6 Actin_HC_7 Actin_NC_7 Actin_SC_7 Actin_HT_7 Actin_NT_7 Actin_ST_7 Actin_HC_8 Actin_NC_8 Actin_SC_8 Actin_HT_8 Actin_NT_8 Actin_ST_8 Logistic_a: 14663.5 15490.5 15836.6 14674.5 15934.4 14176.3 11.2555 17230 16922.7 16376.3 15561.4 14849.5 15583.8 7752.72 16933.2 19225.7 15497.9 16392.4 16720.8 15280.2 3.54E+06 17058.9 18151.8 15541.4 15129.5 16316.7 17325.2 2.00E+06 16068.2 16546.4 15978.5 14980.1 15652.7 14655.6 17509.8 17991.8 15494.1 16096 15249.4 14454.6 14766.8 15168.4 14118.3 14302.5 14886.4 12924.7 13594.9 13612 14560.8 13771.4 12819 12778.1 Logistic_b: -17.2413 -18.5618 -17.1088 -18.9331 -17.9488 -18.2314 -4.74628 -16.7658 -17.1029 -18.0931 -18.4088 -16.9249 -17.8746 -26.7766 -17.6206 -17.3925 -17.0619 -18.4866 -17.5567 -18.565 -19.0691 -16.9321 -17.1269 -17.8324 -17.7005 -18.4337 -18.2413 -15.3095 -17.3046 -17.1332 -17.3935 -18.0897 -18.1654 -18.8144 -19.6836 -16.7216 -16.9756 -18.1482 -18.0908 -17.0919 -16.4606 -18.102 -16.0269 -17.0044 -16.9557 -17.9206 -17.541 -16.077 -16.4225 -17.9358 -17.8167 -18.4089 Logistic_X0: 28.4681 29.9645 28.3426 30.7024 30.1167 29.7038 5.26809 27.5731 28.416 29.1399 30.6104 29.2767 29.2942 38.8208 29.0977 28.6608 28.1917 30.4902 29.4096 31.3718 56.8552 28.0327 28.0829 29.0054 29.332 31.158 30.5925 59.165 28.4552 28.3645 28.7795 30.1051 30.2415 32.0057 33.6027 27.6456 28.3396 30.0464 30.2168 28.3623 27.628 29.7862 26.557 28.6778 28.4876 29.971 28.845 26.4993 27.156 29.7301 30.0975 29.8888 Logistic_Y0: -10.6503 -15.8937 -12.57 -4.844 -21.8322 -5.12097 -8.51771 -9.30453 -14.3043 -11.0975 -13.2714 -25.3453 -11.5071 -1.82994 -5.1697 -12.263 -14.1166 -13.4378 -17.1631 -21.9482 -9.9036 -5.11996 -16.9699 -15.6057 -14.5665 -26.7832 -18.608 -11.5978 -10.9401 -13.2081 -7.46769 -19.9018 -18.6538 -28.0143 -29.6497 -9.79614 -13.7018 -20.9399 -19.0278 -12.0599 -11.6064 -6.71733 -6.49862 -10.7469 -13.1963 -18.8019 -8.76173 -6.50922 -14.1787 -8.57483 -19.096 -14.4827 Logistic_Pvalue: 0 3.33E-16 1.36E-48 0 4.73E-50 0 3.31E-06 2.22E-16 9.43E-50 2.96E-48 0 9.15E-49 3.78E-48 1.11E-16 2.88E-48 1.11E-16 2.10E-48 0 7.63E-49 0 0 0 8.38E-49 1.11E-16 1.20E-49 0 0 0 0 6.05E-49 0 0 0 0 9.37E-49 0 6.67E-49 0 2.22E-50 4.14E-49 0 3.43E-49 0 3.44E-49 0 4.21E-49 0 0 9.74E-45 9.08E-50 2.23E-49 1.11E-16 Noise(SPE): 23.3666 22.1596 23.6202 20.8412 19.8228 21.591 1.52805 26.9734 22.5857 25.5029 20.3027 21.961 24.3012 0.620261 26.4379 29.4815 23.7322 23.9718 24.3102 16.415 320.542 25.3276 26.9607 23.7423 20.5128 18.3406 22.6845 1827.1 25.8486 23.8591 24.3864 20.7544 21.6788 13.1299 13.8676 27.7312 22.4721 23.0091 18.2819 20.5345 23.5715 20.8477 22.7369 19.7924 24.4924 17.7116 21.4034 22.3772 22.6677 18.0163 16.9925 19.8434 EndofExpPhase(SDM): 2670 2846.86 2879.1 2714.62 2908.77 2608.06 Error! 3126.84 3075.51 3004.3 2859.22 2681.53 2852.87 1493.31 3100.63 3507.09 2814.51 3014.25 3047.99 2802.01 Error! 3104.71 3297.98 2839.97 2762.27 2985.69 3175.28 Error! 2928.15 3008.73 2917.5 2738.33 2865.16 2685.59 3232.36 3263.63 2811.98 2944.14 2788.81 2626.86 2668.3 2786.46 2544.53 2598.31 2701.17 2357.71 2483.04 2454.32 2627.37 2523.94 2335.96 2344.22 CP(SPE): 19.5945 21.057 19.3766 21.717 20.7481 20.8123 3.56687 18.7586 19.2983 20.3868 21.3407 19.9227 20.4069 27.2938 20.1662 19.7475 19.2831 21.4212 20.2726 21.7093 34.8957 19.0837 19.2021 20.1652 20.2002 21.5578 21.2619 37.4651 19.6232 19.3638 19.8244 20.9248 21.0511 22.0424 23.3795 18.7713 19.285 20.9446 20.8354 19.3276 18.6843 20.6991 17.7808 19.4739 19.5218 20.7475 19.9673 17.7871 18.3205 20.5339 20.7516 21.0361 CP(SDM): 26.0999 27.662 25.9652 28.3927 27.7175 27.3769 Error! 25.2092 26.0316 26.8384 28.2372 26.7922 26.9501 36.7998 26.7333 26.2989 25.82 28.137 27.0104 28.9616 Error! 25.6549 25.73 26.6785 26.96 28.7459 28.1974 Error! 26.0975 25.9889 26.408 27.7269 27.8632 29.5817 31.1778 25.2687 25.9424 27.681 27.8299 25.9807 25.2119 27.4349 24.1666 26.2563 26.0748 27.5794 26.4897 24.1221 24.7753 27.3599 27.6807 27.5716 DynamicThreshold: 1341.36 1426.56 1445.07 1365.31 1453.38 1312.26 Error! 1572.25 1541.89 1509.35 1433.12 1339.07 1432.83 746.049 1560.95 1762.16 1412.06 1512.39 1527.57 1398.24 Error! 1562.46 1653.99 1424.05 1384.11 1488.63 1589.68 Error! 1471.53 1509.69 1467.21 1369.59 1434.09 1335.35 1608.29 1640.78 1410.38 1473.1 1394.03 1317.67 1340.13 1400.3 1280.38 1303.68 1356.23 1178.31 1247.84 1235.09 1317.93 1266.69 1166.93 1174.79 LowerCycleNumber: 9 9 9 10 10 9 Error! 9 9 9 9 9 9 13 9 9 9 9 9 10 Error! 9 9 9 9 10 9 Error! 9 9 9 9 9 11 11 9 9 9 10 9 9 10 9 9 9 10 9 9 8 10 10 9 UpperCycleNumber: 15 16 16 16 17 15 Error! 15 16 16 16 16 16 20 16 15 16 16 16 17 Error! 16 16 16 16 17 16 Error! 15 16 15 16 16 18 18 15 16 16 17 16 15 16 15 16 15 17 15 15 15 16 17 16 PointsForRegression: 7 8 8 7 8 7 Error! 7 8 8 8 8 8 8 8 7 8 8 8 8 Error! 8 8 8 8 8 8 Error! 7 8 7 8 8 8 8 7 8 8 8 8 7 7 7 8 7 8 7 7 8 7 8 8 Number_OfRegressionWindows: 52 134 143 55 135 57 Error! 56 144 141 145 139 138 87 136 53 134 144 144 145 Error! 135 132 136 138 137 141 Error! 51 137 56 138 141 130 149 58 140 136 135 138 54 56 59 146 50 132 58 59 134 56 132 135 WeightedAverage_OfPvalue: 0.000780786 0.000548161 0.000473429 0.000574329 0.000568838 0.000555279 Error! 0.000392036 0.000430139 0.000530031 0.000387499 0.000616184 0.00049582 0.00013548 0.000655823 0.000433579 0.000638582 0.000429321 0.00049044 0.000452363 Error! 0.000881893 0.000761597 0.000650468 0.000478321 0.000528412 0.000387866 Error! 0.000693643 0.000478349 0.000378312 0.000561637 0.00052401 0.000582121 0.000375803 0.000375641 0.000532293 0.000564324 0.000523712 0.000503119 0.000452732 0.00052044 0.000405141 0.00033012 0.000895428 0.000636222 0.000374571 0.000427787 0.000664335 0.000643678 0.000597533 0.000583068 Stderr_OfWeightedPvalue: 0.0021923 0.000967985 0.00104298 0.00208283 0.00107238 0.00215812 Error! 0.000756682 0.00086853 0.00116472 0.00105771 0.000974171 0.00117962 0.000627256 0.000903174 0.00140125 0.000924975 0.00110809 0.00113221 0.00106775 Error! 0.00107743 0.000864513 0.00090664 0.00111029 0.00118441 0.00102793 Error! 0.00184504 0.00103267 0.000527677 0.00104635 0.0011194 0.00148418 0.00102497 0.00070227 0.0010024 0.000965582 0.00128451 0.00104819 0.000820179 0.00187561 0.000835395 0.000615794 0.00254841 0.00119807 0.00141389 0.000881511 0.000959102 0.00238218 0.00112904 0.000869524 WeightedAverage_OfEfficiency: 0.855179 0.850319 0.858074 0.877181 0.847657 0.869518 Error! 0.85308 0.86114 0.858008 0.875611 0.848946 0.859279 1.02981 0.848093 0.866001 0.852504 0.870458 0.861798 0.864856 Error! 0.846098 0.852304 0.846661 0.863135 0.851301 0.876612 Error! 0.863101 0.858185 0.866791 0.848945 0.858084 0.842244 0.882897 0.855828 0.855141 0.846349 0.855361 0.857077 0.854842 0.877368 0.847275 0.873799 0.846922 0.836662 0.879469 0.851371 0.839838 0.872519 0.840994 0.842278 Stderr_OfWeightedEfficiency: 0.00999203 0.00607454 0.00524795 0.0074272 0.00597268 0.00716649 Error! 0.0084943 0.00550709 0.00565944 0.00482664 0.00603949 0.00542397 0.00968245 0.00629284 0.00937001 0.00634944 0.00545826 0.00554701 0.00507903 Error! 0.00683957 0.0070183 0.00610841 0.00564463 0.005945 0.00525118 Error! 0.0102363 0.00581623 0.00803255 0.00586641 0.00613454 0.00540701 0.00468888 0.0096403 0.00583056 0.0057103 0.00600086 0.00580776 0.0104413 0.00804214 0.0104222 0.00574116 0.00837505 0.00587864 0.00748244 0.0111787 0.00648696 0.00896514 0.00556194 0.00571595 CT: 24.9316 26.5065 24.7935 27.2288 26.5203 26.2143 Error! 24.05 24.855 25.6906 27.0472 25.5705 25.7841 35.7093 25.5608 25.1309 24.652 26.9574 25.8208 27.7488 Error! 24.4855 24.5699 25.5215 25.7809 27.5344 26.9987 Error! 24.9334 24.8172 25.2351 26.5395 26.6747 28.3571 29.9409 24.1037 24.7625 26.4995 26.6365 24.8065 24.0325 26.2607 23.0072 25.0635 24.8893 26.3875 25.323 22.9685 23.6134 26.1779 26.4774 26.4118 TotalSampleNumber: 52 ReplicateSampleNames: Actin_HC Actin_NC Actin_SC Actin_HT Actin_NT Actin_ST Actin_NTC AverageEfficiency_OfReplicateSamples 0.860345 0.859065 0.853037 0.866 0.851383 0.856066 1.02981 StdErr_OfReplicateSamplesEfficiency: 0.00490037 0.00321842 0.00296421 0.00427999 0.00237871 0.0050875 0 CoefficientVariation(CV%)_OfReplicateSamplesEfficiencies: 1.61102 1.05965 0.982848 1.39788 0.790245 1.6809 0 AverageCT_OfReplicateSamples: 25.4072 24.9015 24.6595 26.4118 26.2655 26.5886 35.7093 StdErr_OfReplicateSamplesCT: 0.676345 0.400178 0.327071 0.255775 0.2866 0.393627 0 CV%_OfReplicateSamplesCTs: 7.52933 4.5454 3.75148 2.73907 3.08629 4.1873 0 GeneNames Actin AverageEfficiency_OfGenes: 0.882244 Stdev_OfGenesEfficiency: 0.0246638 MeanCV%_OfGenesEfficiencies 1.07465 MeanCV%_OfGenesCTs 3.69127 ReplicateSampleNames: Actin_HC Actin_NC Actin_SC Actin_HT Actin_NT Actin_ST Actin_NTC 1.05016E-07 1.44596E-07 1.68511E-07 5.56307E-08 6.10238E-08 4.97453E-08 1.55423E-10 "Some problems are found! ------Please check your data and re-run Realtime PCR Miner (Version 4.0, Author: Dr. Sheng Zhao, Email: windupzs@gmail.com)." "Error#1: Sample#7:Actin_NTC_1, Exponential phase fit failed!" "Error#2: Sample#21:Actin_NTC_3, Exponential phase fit failed!" "Error#3: Sample#28:Actin_NTC_4, Exponential phase fit failed!"