#Quarter Project Mon-Wed Thermal Shock Project
##Baseline
Animals kept at 8°C for 3 weeks prior to experiment fed on a daily basis. 
##Experiment
15 Dabob and 15 Oyster bay oysters in a closed treatment aquarium system. 

The temperature will gradually be raised from 8 to 35°C over one hour. 

The animals will then be held at 35°C for 1 hour. 

Then the tanks will be allowed to gradually lower temperature until they reach 8°C.
 
##Sample collection
After 3 hour treatment 5 oysters from each group will be sampled and placed into RNAlater for RNA isolation. 

##Team Measurements
**The Olympia Oysters**
HSP70 expression

HSP90

**Cuttlefish**
Mortality (1 week)

Caspase

**Fuzzy Chiton**
Metabolic Rate
(O2 consumption)

citrate synthase

**Crabs**
Recovery of 'normal' metabolism

Housekeeping Gene Concentrations

candidate gene: glutamine synthetase

**Immortal Jellyfish**
Return to Baseline metabolism
Test oysters once an hour for the first 6 hours
Test again after 24 hours

citrate synthase

## Treatment details
we provide ambient and hot 35°C water, and buckets
Students figure out how to manage treatment over the class period.
end with tissue/ RNA samples from 5 oysters from each group.

need:
temperature logger
buckets
hot seawater
thermometer

Other:
QPR background provided to student for next lab.
QPR standardization procedure.