##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	olyo-rad-01.fastq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	222197679	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.580181001800653	31.0	31.0	34.0	27.0	34.0
2	30.705166456756732	31.0	31.0	34.0	27.0	34.0
3	30.772638718696967	31.0	31.0	34.0	27.0	34.0
4	34.1247917895668	37.0	35.0	37.0	30.0	37.0
5	34.11300456923315	37.0	35.0	37.0	30.0	37.0
6	33.76274678368715	37.0	35.0	37.0	29.0	37.0
7	33.64343688756533	37.0	35.0	37.0	28.0	37.0
8	33.5780847737838	37.0	35.0	37.0	28.0	37.0
9	34.97812912798248	39.0	35.0	39.0	28.0	39.0
10-14	35.07867676601608	39.2	35.2	39.4	27.0	39.4
15-19	35.871221137282895	40.0	36.0	41.0	27.0	41.0
20-24	35.759425217038384	40.0	36.0	41.0	27.0	41.0
25-29	35.55539643958207	40.0	35.8	41.0	25.4	41.0
30-34	35.2958473378113	40.0	35.0	41.0	24.4	41.0
35-39	35.05504315461369	40.0	35.0	41.0	23.8	41.0
40-44	34.751167501619136	39.0	34.6	41.0	22.2	41.0
45-49	34.39713553263533	39.0	34.0	41.0	19.2	41.0
50-54	33.98927795190876	39.0	33.4	41.0	15.8	41.0
55-59	33.474318550375145	38.0	33.0	40.4	10.4	41.0
60-64	32.8165071787271	37.2	32.2	40.0	6.0	41.0
65-69	32.032467799089844	36.2	31.4	39.4	2.0	41.0
70-74	31.159126722471296	35.0	31.0	38.4	2.0	40.4
75-79	29.187041023052274	33.8	28.6	35.8	2.0	38.6
80-84	29.56691672553429	35.0	30.0	36.0	2.0	37.6
85-89	28.928390666943017	34.4	29.4	35.0	2.0	36.4
90-94	28.351194492900174	34.0	29.0	35.0	2.0	36.0
95-99	27.74624219184576	34.0	29.0	35.0	2.0	35.2
100-101	26.246145030614834	33.0	24.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5840345.0
3	2326326.0
4	1103894.0
5	873754.0
6	1031037.0
7	778444.0
8	719056.0
9	824588.0
10	956399.0
11	961218.0
12	1005172.0
13	1077473.0
14	1140190.0
15	1189535.0
16	1239236.0
17	1270905.0
18	1214065.0
19	1182614.0
20	1171383.0
21	1201544.0
22	1297845.0
23	1464597.0
24	1713619.0
25	2045575.0
26	2450504.0
27	2921516.0
28	3383033.0
29	3971324.0
30	4689638.0
31	5594945.0
32	6793338.0
33	8430467.0
34	1.0909816E7
35	1.4712478E7
36	2.2469336E7
37	3.8958538E7
38	4.8636557E7
39	1.4616011E7
40	31364.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.326114762393715	16.670684733974266	18.140019988696043	34.86318051493598
2	23.740551519412968	26.730897915332513	22.351814924413393	27.176735640841127
3	21.586268733854595	25.92072285254997	22.887360729060653	29.60564768453478
4	21.280083747100317	26.029805265766097	24.268337095725638	28.42177389140795
5	22.063096174114822	30.423176210900454	24.93543185214181	22.57829576284291
6	21.949343377191006	29.90075925707612	22.419364064116323	25.730533301616553
7	21.583557978560837	26.281033431231887	32.43754457675656	19.69786401345072
8	30.428572186198267	25.745789822553334	23.36391385840553	20.461724132842868
9	19.92054970034145	24.914568987060978	23.300765304968387	31.864116007629185
10-14	22.59462158431078	31.619114598990038	25.368197334743943	20.41806648195524
15-19	19.6430403335057	30.98143367570559	29.47697238188402	19.89855360890469
20-24	19.5396248494638	30.600493536170898	30.02423141773872	19.835650196626588
25-29	19.204976289220916	30.166933152888824	30.7858222967842	19.842268261106064
30-34	19.342093177120002	30.401227961753918	30.181682395725385	20.074996465400694
35-39	19.305485786116357	30.359733880387534	30.23825440838539	20.096525925110715
40-44	19.57205330355669	30.10115027431818	30.29269293487734	20.034103487247794
45-49	19.37196995782113	29.891854072866437	30.51977800896558	20.21639796034686
50-54	19.304744736801567	30.3630427671573	30.27472499341871	20.057487502622422
55-59	19.367620410349176	29.85022254425876	30.594822559992323	20.18733448539974
60-64	19.422858127182543	30.221933721023063	30.521535033362373	19.833673118432017
65-69	19.474402135181553	30.202394412232366	30.339671261901636	19.98353219068445
70-74	19.164950070817998	30.349430060817127	30.519054620552353	19.966565247812515
75-79	19.42070500381538	29.985642188288793	30.388330979468726	20.205321828427095
80-84	19.519418770266608	30.08522201128283	30.40643694930284	19.988922269147725
85-89	19.52852739892402	30.21435850386108	30.423995164105815	19.83311893310908
90-94	19.699355292983718	30.408624718564862	30.106307749284028	19.785712239167392
95-99	19.314513339704302	30.739323810078425	30.136563735040472	19.809599115176805
100-101	19.632764687200446	30.157943160742747	30.609044047404616	19.6002481046522
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.18929527732969
2	50.91728716025409
3	51.19191641838938
4	49.701857638508265
5	44.64139193695773
6	47.67987667880756
7	41.28142199201155
8	50.89029631904114
9	51.78466570797063
10-14	43.01268806626602
15-19	39.541593942410394
20-24	39.37527504609039
25-29	39.04724455032698
30-34	39.4170896425207
35-39	39.402011711227075
40-44	39.606156790804484
45-49	39.58836791816799
50-54	39.36223223942399
55-59	39.55495489574892
60-64	39.25653124561456
65-69	39.457934325866
70-74	39.13151531863052
75-79	39.62602683224248
80-84	39.50834103941433
85-89	39.361646332033104
90-94	39.485067532151106
95-99	39.12411245488111
100-101	39.233012791852644
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1755.0
1	6484.5
2	18253.5
3	27588.0
4	26697.0
5	19909.5
6	14890.0
7	13406.0
8	14078.5
9	15763.5
10	18293.5
11	22077.5
12	27097.5
13	33599.5
14	41317.0
15	51065.5
16	63362.0
17	79671.5
18	105351.0
19	144834.0
20	207058.5
21	302738.0
22	442991.5
23	643310.5
24	913232.0
25	1264493.0
26	1716650.0
27	2269908.5
28	2929706.5
29	3711914.5
30	4602909.5
31	5596039.5
32	6690188.0
33	7835883.0
34	8935641.5
35	9812441.5
36	1.0359779E7
37	1.0705082E7
38	1.09138785E7
39	1.09084055E7
40	1.0747606E7
41	1.05129155E7
42	1.01753995E7
43	9691873.0
44	9132633.5
45	8534860.5
46	7880036.5
47	7240748.0
48	6617639.0
49	6000003.5
50	5388924.0
51	4764826.0
52	4122894.0
53	3521791.5
54	3003779.5
55	2542358.0
56	2136703.0
57	1789100.5
58	1477320.5
59	1190224.5
60	936851.5
61	711034.5
62	522492.5
63	390803.0
64	308684.5
65	255468.5
66	214079.0
67	175557.0
68	143527.0
69	116890.5
70	94093.5
71	73784.5
72	57541.0
73	44150.0
74	32662.5
75	25454.0
76	19783.0
77	15258.0
78	12516.5
79	10441.5
80	8756.5
81	7578.0
82	6555.5
83	5601.0
84	4914.0
85	4319.5
86	3718.5
87	3140.5
88	2727.5
89	2340.0
90	1891.5
91	1567.5
92	1303.5
93	1025.5
94	810.5
95	655.0
96	480.5
97	331.0
98	211.5
99	105.0
100	51.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15179456487482032
2	0.03008942321130186
3	0.004780878021682666
4	0.009948798790107974
5	0.02939724676422025
6	0.1900712923288456
7	0.21115252063456524
8	0.2037064482568245
9	0.21940013153782761
10-14	0.1963734283651091
15-19	0.21470350282101733
20-24	0.30799169598886766
25-29	0.30289722333238234
30-34	0.32592662680333395
35-39	0.3427502948849434
40-44	0.3518172662820659
45-49	0.2906477704476832
50-54	0.19366250895897072
55-59	0.18998992334208856
60-64	0.20873476360659915
65-69	0.22259539443704088
70-74	0.22436471984930137
75-79	0.2166096433437543
80-84	0.21491772648084229
85-89	0.22481233928640632
90-94	0.22197369577384288
95-99	0.2901546959903213
100-101	0.34406637523878003
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.22197679E8
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	46.473620494396066
#Duplication Level	Relative count
1	100.0
2	28.56400248057491
3	9.634115200817131
4	4.303615073140481
5	2.5307335935505053
6	1.807536570240397
7	1.4609856637361835
8	1.2147521249042426
9	1.1153467333017182
10++	31.764126509320395
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GGGGG	34569675	5.0768166	12.961188	1
CCCCC	35530795	4.4639106	17.183435	2
GCAGG	32718125	3.1033363	159.08586	8
TGCAG	38117465	2.447543	111.360596	7
CAGGG	22268760	2.112207	34.152065	9
GGGGC	14626665	2.0820174	5.879652	1
CACAC	33357745	1.9807256	5.125629	1
GCCCC	14374595	1.8632162	5.0248523	1
CCCCT	21167335	1.8573754	8.354749	3
GGTCA	28667155	1.8407334	5.6018405	1
CCCTG	19453380	1.7611071	7.595264	4
CCCCA	20297580	1.7531271	6.2006283	1
GGCCC	13095770	1.751281	5.704191	1
CTGCA	27744475	1.726735	41.114086	6
ACCCC	19974285	1.7252035	8.461804	1
CAGGT	25880725	1.6618152	34.99436	9
CAGGA	25878265	1.6355997	30.622873	9
CACTG	26038325	1.6205492	6.2747746	4
CCTGC	16402320	1.4848956	12.943062	5
TGCTG	22718410	1.4820015	6.0660205	4
ATGCA	34625415	1.4815037	22.054665	6
CCATG	23487400	1.4617871	7.338468	2
CCCTA	23969420	1.4459379	5.5938563	1
CTGTG	22006005	1.4355289	5.097043	4
GTCTG	21998790	1.4350582	7.9052124	4
CCCCG	10827840	1.4034905	5.182749	1
TTGCA	32179075	1.398768	17.651691	6
GTGCA	21726885	1.395095	30.79665	6
GGCCA	15136145	1.3915495	5.558931	1
CCCGG	10058680	1.3451347	5.437222	1
CAGGC	14399365	1.3238131	32.954582	9
TTTGC	29311920	1.2944367	5.3417864	5
TGCAT	29432205	1.2793664	5.3541336	7
ATGTG	28444080	1.2756195	5.478102	4
TCTGC	20164275	1.274959	10.663267	5
CATGC	20016835	1.2457892	8.746203	5
TGTGC	18650220	1.2166191	15.98745	5
TGCAC	18687900	1.1630803	6.6805854	7
GCTGC	12307735	1.1495447	12.836295	5
GGTGC	11891810	1.1459161	10.005082	5
ACTGC	18375520	1.1436385	16.886597	5
AATGC	25906930	1.1084694	8.793271	5
CGGAC	11713275	1.0768661	5.922155	2
GATGC	16415800	1.0540673	9.657923	5
CATAC	24707860	1.0246739	5.5346513	2
GTTGC	15447485	1.0076935	5.2108016	5
ATTGC	22960085	0.9980347	8.799217	5
CCGAG	10690375	0.9828253	5.49701	1
CGCCA	10791750	0.96165234	5.521156	2
GCTCA	15374500	0.9568639	5.190286	1
CGTGC	9254385	0.8643612	7.8801403	5
AGTGC	13155880	0.84474605	6.127049	5
CGCTG	8971935	0.83798033	5.6876216	2
TCGCC	8909980	0.80661696	5.0569296	1
CCGCA	8416055	0.74995434	7.506189	1
GCGCT	6521700	0.609128	5.445375	1
>>END_MODULE