FastQCFastQC Report
Wed 10 Dec 2014
olyo-rad-01.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename olyo-rad-01.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 222197679
Filtered Sequences 0
Sequence length 101
%GC 40

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 34569675 5.0768166 12.961188 1
CCCCC 35530795 4.4639106 17.183435 2
GCAGG 32718125 3.1033363 159.08586 8
TGCAG 38117465 2.447543 111.360596 7
CAGGG 22268760 2.112207 34.152065 9
GGGGC 14626665 2.0820174 5.879652 1
CACAC 33357745 1.9807256 5.125629 1
GCCCC 14374595 1.8632162 5.0248523 1
CCCCT 21167335 1.8573754 8.354749 3
GGTCA 28667155 1.8407334 5.6018405 1
CCCTG 19453380 1.7611071 7.595264 4
CCCCA 20297580 1.7531271 6.2006283 1
GGCCC 13095770 1.751281 5.704191 1
CTGCA 27744475 1.726735 41.114086 6
ACCCC 19974285 1.7252035 8.461804 1
CAGGT 25880725 1.6618152 34.99436 9
CAGGA 25878265 1.6355997 30.622873 9
CACTG 26038325 1.6205492 6.2747746 4
CCTGC 16402320 1.4848956 12.943062 5
TGCTG 22718410 1.4820015 6.0660205 4
ATGCA 34625415 1.4815037 22.054665 6
CCATG 23487400 1.4617871 7.338468 2
CCCTA 23969420 1.4459379 5.5938563 1
CTGTG 22006005 1.4355289 5.097043 4
GTCTG 21998790 1.4350582 7.9052124 4
CCCCG 10827840 1.4034905 5.182749 1
TTGCA 32179075 1.398768 17.651691 6
GTGCA 21726885 1.395095 30.79665 6
GGCCA 15136145 1.3915495 5.558931 1
CCCGG 10058680 1.3451347 5.437222 1
CAGGC 14399365 1.3238131 32.954582 9
TTTGC 29311920 1.2944367 5.3417864 5
TGCAT 29432205 1.2793664 5.3541336 7
ATGTG 28444080 1.2756195 5.478102 4
TCTGC 20164275 1.274959 10.663267 5
CATGC 20016835 1.2457892 8.746203 5
TGTGC 18650220 1.2166191 15.98745 5
TGCAC 18687900 1.1630803 6.6805854 7
GCTGC 12307735 1.1495447 12.836295 5
GGTGC 11891810 1.1459161 10.005082 5
ACTGC 18375520 1.1436385 16.886597 5
AATGC 25906930 1.1084694 8.793271 5
CGGAC 11713275 1.0768661 5.922155 2
GATGC 16415800 1.0540673 9.657923 5
CATAC 24707860 1.0246739 5.5346513 2
GTTGC 15447485 1.0076935 5.2108016 5
ATTGC 22960085 0.9980347 8.799217 5
CCGAG 10690375 0.9828253 5.49701 1
CGCCA 10791750 0.96165234 5.521156 2
GCTCA 15374500 0.9568639 5.190286 1
CGTGC 9254385 0.8643612 7.8801403 5
AGTGC 13155880 0.84474605 6.127049 5
CGCTG 8971935 0.83798033 5.6876216 2
TCGCC 8909980 0.80661696 5.0569296 1
CCGCA 8416055 0.74995434 7.506189 1
GCGCT 6521700 0.609128 5.445375 1